Effects of exosomes secreted by mesenchymal stem cells mediated by astragaloside IV on biological function and pyroptosis of damaged endothelial cells induced by high glucose
10.3760/cma.j.cn431274-20211020-01096
- VernacularTitle:黄芪甲苷介导间充质干细胞外泌体对高糖诱导损伤的内皮细胞生物学功能和细胞焦亡的影响
- Author:
Wu XIONG
1
;
Meixin TAN
;
Zilin CHEN
;
Fanxin OUYANG
;
Luyao ZHANG
;
Qianpei YANG
;
Ajian PENG
;
Wenfei LIANG
;
Xi ZHANG
Author Information
1. 湖南中医药大学第一附属医院烧伤整形外科，长沙 410007
- Keywords:
Astragaloside Ⅳ;
Mesenchymal stem cells;
Exosomes;
Endothelial cells;
Pyroptosis
- From:
Journal of Chinese Physician
2021;23(12):1769-1773,1781
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of mesenchymal stem cells-derived exosomes (MSC-Exos) secreted by mesenchymal stem cells (MSCs) induced by astragaloside IV (AS-IV) on the biological function and pyroptosis of human umbilical vein endothelial cells (HUVECs) injured by high glucose.Methods:After human umbilical cord blood mesenchymal stem cells (hUCBMSCs) were intervened with 400 mg/L of AS-IV, exosomes were extracted, and then the morphology and specific markers of exosomes were identified. Human umbilical vein endothelial cells (HUVECs) were cultured in a medium with a glucose concentration of 30 mmol/L to prepare a high glucose-impaired HUVECs model. High glucose-impaired HUVECs were randomly divided into experimental and model groups, with the experimental group intervened with 100 μg/ml of MSC-Exos and the model group intervened with an equal volume of PBS solution, while a blank control group was also set up. Cell counting Kit-8 (CCK-8) cell proliferation assay, adhesion assay, matrigel tube formation assay and scratch assay were used to detect the effects of AS-IV-mediated MSC-Exos on the proliferation, adhesion, tube formation and migrationability of HUVECs; Western blot and real time fluorescence quantitative polymerase chain reaction (qRT-PCR) were used to detect the protein and mRNA expression of scorch death-related molecules, such as Caspase-1, GSDMD (Gasdermin D) and NLRP3 in each group.Results:The proliferation, adhesion number, tube number and migration width of HUVECs cells were significantly lower than those in the blank group ( P<0.05); The expression of Caspase-1, GSDMD, NLRP3 protein and their mRNA increased significantly ( P<0.001); Under the intervention of MSC-Exos mediated by AS-IV, the cell proliferation, adhesion number, tube number and migration width of HUVECs were significantly higher than those in the model group ( P<0.05); The expression of Caspase-1, GSDMD, NLRP3 protein and their mRNA decreased, with statistically significant difference ( P<0.05). Conclusions:AS-IV mediated MSC-Exos can significantly improve the biological function of high glucose-impaired endothelial cells, and its mechanism may be related to anti-pyroptosis.
