Establishment of LINC01018 overexpression colon cancer cell line and study on its biological characteristics
10.3760/cma.j.cn431274-20210530-00606
- VernacularTitle:LINC01018过表达结肠癌细胞株的建立及其生物学特性
- Author:
Hongwei CHEN
1
;
Jiahong CAI
;
Xiaochen ZENG
;
Bingjie PAN
;
Zhoujing LIU
;
Songtao CHEN
Author Information
1. 湖南省脑科医院（湖南省第二人民医院）普外科，长沙 410007
- Keywords:
Colonic neoplasms;
RNA, long noncoding;
LINC01018 genes;
HT29 cells
- From:
Journal of Chinese Physician
2021;23(11):1663-1667
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a colon cancer cell line which overexpressing LINC01018 stably and study its biological characteristics.Methods:The expression of LINC01018 in HCoEpiC and HT-29 cells were detected by real time fluorescence quantitative polymerase chain reaction (qRT-PCR). HT-29 cells were infected with LINC01018 overexpression lentivirus to screen and establish HT-29 cell lines which overexpressing LINC01018 stably. The effect of LINC01018 on the proliferation, invasion and migration of HT-29 cells were detected by cell counting kit-8 (CCK-8) assay and Transwell assay separately. The expression of CDK6 and matrix metalloproteinase-2 (MMP-2) in HT-29 cells was detected by Western blot.Results:The expression of LINC01018 in HT-29 cells was significantly lower than that in the human colonic epithelial cells (HCoEpiC). HT-29-L18 cell lines which overexpressing LINC01018 stably was screened successfully. Overexpression of LINC01018 significantly inhibited the cell proliferation, invasion and migration, and reduced the protein expression of CDK6 and MMP-2 in HT-29 cells.Conclusions:The expression of LINC01018 was decreased abnormally in colon cancer cells. Up-regulation of LINC01018 expression can inhibit the proliferation, invasion and migration of colon cancer cells, which may be related to CDK6 and MMP-2.
