Optogenetic study of glutamatergic neuron in parasubiculum in visuospatial memory
10.3760/cma.j.cn371468-20210823-00480
- VernacularTitle:海马旁下托谷氨酸能神经元在视空间记忆中的光遗传研究
- Author:
Xinyang QI
1
;
Yu SONG
;
Xingjian LIN
Author Information
1. 南京医科大学附属脑科医院神经内科 210029
- Keywords:
Spatial memory;
Parasubiculum;
Glutamatergic neuron;
Optogenetics;
Mice
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2021;30(12):1064-1068
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the function of glutamatergic neuron of the parasubiculum in spatial memory.Methods:Sixteen adult male C57BL/6 mice aged 6-8 weeks were divided into two groups randomly, 0.4 μl AAV5-CaMKⅡα-eNpHR3.0-eYFP was injected into the bilateral parasubiculum respectively in experimental group, equal dose AAV5-CaMKⅡα-eYFP for control group.The optic fiber was implanted 6 weeks after virus injection.The novel object place recognition test was performed one week after optic fiber implantation, continuous yellow light was delivered during the behavioral test to inhibit the function of glutamatergic neuron in the parasubiculum.The standard memory index (D2) was used to evaluate the spatial memory function.SPSS 20.0 software was used to process the data, and the independent-samples t-test and paired-samples t-test were used for data analysis. Results:In the novel object place recognition experiment, the mice showed no preference for either object in both control group(new object: 0.51±0.06, familiar object: 0.49±0.04, t=1.21, P>0.05) and experimental group(new object: 0.49±0.05, familiar object: 0.50±0.04, t=-0.78, P>0.05). Compared with the control group (0.55±0.06), the D2 score of the experimental group (0.26±0.07) was significantly lower ( t=-2.96, P<0.05), and the number of c-fos positive neuron in experimental group (96.33±7.13) was also significantly less than that in control group (127.67±5.24, t=-3.54, P<0.05). Conclusion:Inhibiting glutamatergic neuronal activity in the parasubiculum impairs spatial memory in mice, suggesting that glutamatergic neurons of the parasubiculum play an important role in spatial memory.
