Anti-Inflammatory Effects of Chrysanthemum indicum Water Extract in RAW 264.7 Cell as a Whole Plant.
10.7586/jkbns.2015.17.4.341
- Author:
Kyoungah KANG
1
Author Information
1. Department of Nursing, Kunsan National University, Gunsan, Korea. rallycar@kunsan.ac.kr
- Publication Type:Original Article
- Keywords:
Anti-inflammation;
Chrysanthemum indicum;
RAW 264.7 cell
- MeSH:
Chrysanthemum*;
Dinoprostone;
Down-Regulation;
Interleukins;
Korea;
Lipopolysaccharides;
Macrophages;
Nitric Oxide;
Nitric Oxide Synthase Type II;
Plants*;
Prostaglandin-Endoperoxide Synthases;
RNA;
Water*
- From:Journal of Korean Biological Nursing Science
2015;17(4):341-347
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Chrysanthemum indicum (CHI) has been used for edible and medical purposes for a long time in Korea. The purpose of this study was to evaluate the anti-inflammatory effects of CHI water extract in lipopolysaccharides (LPS)-induced RAW 264.7 macrophage cells. METHODS: To investigate the anti-inflammatory effects on LPS-induced RAW 264.7 macrophage cells, CHI extract as a whole plant was used in this study. RAW 264.7 cells were treated with various concentrations of CHI extract (1, 10, and100 microg/mL). After that Nitric Oxide (NO), inducible nitric oxide synthase (iNOS), interleukin (IL)-1beta, cyclooxygenase (COX)-2 and prostaglandin E2 (PGE2) expression level were measured. RESULTS: CHI extract significantly suppressed the LPS-induced NO production and decreased the level of iNOS, IL-1beta, COX-2 messenger ribonucleic acid (mRNA) expression and also the down regulation of PGE2 expression in a dose-dependent manner. CONCLUSION: The present study suggested that CHI extract can be substituted for anti-inflammatory drugs and provide a safe and effective non pharmacological therapeutic approach.
