Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography
- Author:
Peijie ZHU
1
,
2
;
Weijia CHEN
;
Qiqin WANG
;
Huihui WU
;
Meng RUAN
;
Hongwu WANG
;
Zhengjin JIANG
Author Information
1. School of Food&Pharmaceutical Engineering,Zhaoqing University,Zhaoqing,Guangdong,526061,China
2. Institute of Pharmaceutical Analysis,College of Pharmacy,Jinan University,Guangzhou,510632,China
- Keywords:
Phosphatidylethanolamine;
Biomimetic monolith;
IAM;
Drug-membrane interaction
- From:
Journal of Pharmaceutical Analysis
2022;12(2):332-338
- CountryChina
- Language:Chinese
-
Abstract:
In this research,a new phospholipid based monolith was fabricated by in situ co-polymerization of 1-dodecanoyl-2-(11-methacrylamidoundecanoyl)-sn-glycero-3-phosphoethanolamine and ethylene dimethacrylate to mimick bio-membrane environment.Excellent physicochemical properties of this novel monolith that were achieved included column efficiency,stability,and permeability.Moreover,the biomimetic monolith showed outstanding separation capability for a series of intact proteins and small molecules.In particular,it exhibited good potential as an alternative to the commercial immobilized artificial membrane(IAM)column(IAM.PC.DD2)for studying drug-membrane interactions.This study not only enriched the types of IAM stationary phases,but also provided a simple model for the prediction of phosphatidylethanolamine related properties of drug candidates.