Hyphenated LC-ABTS+and LC-DAD-HRMS for simultaneous analysis and identification of antioxidant compounds in Astragalus emarginatus Labill.extracts
- Author:
Chamandy ABIR
1
,
2
;
Zhao MINJIE
;
Rammal HASSAN
;
Ennahar SA?D
Author Information
1. Analytical Chemistry of Bioactive Molecules and Pharmacognosy Team(CAMBAP),Hubert Curien Multidisciplinary Institute(IPHC),National Center for Scientific Research(CNRS),Faculty of Pharmacy,University of Strasbourg,Illkirch,67400,France
2. Research Platform of Analysis and Environmental Science(PRASE),Lebanese University,Hadath,005,Lebanon
- Keywords:
Antioxidant;
Astragalus emarginatus;
Catechol;
Flavonoids;
Hydroxycinnamates;
Caffeic acid;
Quercetin
- From:
Journal of Pharmaceutical Analysis
2022;12(2):253-262
- CountryChina
- Language:Chinese
-
Abstract:
The compounds in leaf and stem extracts of Astragalus emarginatus Labill.(AEL),a plant species used in traditional Lebanese medicine,were investigated for antioxidant properties.First,the activity of various extracts was assessed using the Trolox equivalent antioxidant capacity,oxygen radical absorption ca-pacity,and 2,2-diphenyl-1-picryl-hydrazy l-hydrate assays.The extract obtained using 30％ethanol showed the greatest activity.The antioxidant compounds in this extract were screened using a hy-phenated high-performance liquid chromatography-2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate)radical(ABTS+)system before being separated by ultra-high-performance liquid chromatography and identified using high-resolution mass spectrometry and ultra-violet-visible diode array detection.Approximately 40 compounds were identified.Hydroxycinnamates(caffeic,ferulic,and p-coumaric acid derivatives)and flavonoids(quercetin,luteolin,apigenin,and isorhamnetin derivatives)were the two main categories of the identified compounds.The active compounds were identified as caffeic acid de-rivatives and quercetin glycosides.In addition,the catechol moiety was shown to be key to antioxidant activity.This study showed that AEL is a source of natural antioxidants,which may explain its medicinal use.
