Simultaneous determination of 35 constituents and elucidation of effective constituents in a multi-herb Chinese medicine formula Xiaoer-Feire-Kechuan
- Author:
Zhanpeng SHANG
1
;
Lulu XU
;
Yi KUANG
;
Yan LIN
;
Shuang LIU
;
Long SUN
;
Tao BO
;
Min YE
;
Xue QIAO
Author Information
1. State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China
- Keywords:
Multi-herb formulas;
Effective constituents;
Xiaoer-Feire-Kechuan formula (XFK);
Parallel reaction monitoring (PRM);
Cyclooxygenase-2 (COX-2)
- From:
Journal of Pharmaceutical Analysis
2021;11(6):717-725
- CountryChina
- Language:Chinese
-
Abstract:
Xiaoer-Feire-Kechuan (XFK) is an 11-herb Chinese medicine formula to treat cough and pulmonary inflammation.The complicated composition rendered its chemical analysis and effective-component elucidation.In this study,we combined quantitative analysis and bioactivity test to reveal the anti-inflammatory constituents of XFK.First,UPLC-DAD and UHPLC/Q-Orbitrap-MS methods were estab-lished and validated to quantify 35 analytes (covering 9 out of 11 herbs) in different XFK formulations.Parallel reaction monitoring mode built in Q-Orbitrap-MS was used to improve the sensitivity and selectivity.Then,anti-inflammatory activities of the 35 analytes were analyzed using in vitro COX-2 inhibition assay.Finally,major analytes forsythosides H,I,A (8-10),and baicalin (15) (total contents varied from 21.79 to 91.20 mg/dose in different formulations) with significant activities (inhibitory rate ≥ 80％) were proposed as the anti-inflammatory constituents of XFK.The present study provided an effective strategy to discover effective constituents of multi-herb formulas.
