Tyrosine Protein Kinase (trk) Receptors Expression in Malignant Melanoma Cells.
- Author:
Jung Youl LEE
1
;
Young Lip PARK
;
Kyu Uang WHANG
Author Information
1. Department of Dermatology, College of Medicine, Soonchunhyang University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Neutrophic factors;
Melanoma cells;
Tyrosine protein kinase(trk) receptors
- MeSH:
Biology;
Brain;
Cell Line;
Cell Proliferation;
Cell Survival;
Ectoderm;
Flow Cytometry;
Immunoblotting;
Intercellular Signaling Peptides and Proteins;
Melanoma*;
Nerve Growth Factor;
Nerve Growth Factors;
Neurons;
Protein-Tyrosine Kinases*;
Receptor, trkB;
Sensitivity and Specificity;
Tyrosine*
- From:Korean Journal of Dermatology
1997;35(6):1151-1158
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Nerve growth factor(NGF), brain derived neurotrophic factor(BDNF), neurotropin 3(NT-3) and neurotropir-4/5 are neurotrophic factors necessary for the development and maintenance of specific neurors. The tyrosine protein kinase(trk) receptors exhibit specificity for differ ent neurotrophins. NGF is the cognate ligand for the trk A receptor, BDNF binds to trk B receptor and NT-3 binds to irk A, trk B and trk C receptors, Since melanoma cells are devived from neural ectoderm, growth factors which affect neuronal tissue may have a role in melanoma biology. OBJECTIVE: The purpose of this study is to demonstrate the presence of trk receptors in rnelanoma cells and observe th effect of K-252a on these melanoma cells growth and differentiation. METHODS: After K252a over a range of 0-200nM was added into their cell lines, we exam ined cell viability of SK 28 and SK 30 cells. We performed this to examine the expression of the trk by flow cytometry and immunoblotting. RESULTS: 1. The incubation of SK 28 cells and SK 30 cells with K 252a resulted in a dose dependent inhibition of cell proliferation. 2. In the flowcytometry, SK 28 cells and SK 30 cells showed a high expression of trk A and trk B, not trk C. 3. Using immunoblottiiig, trk in SK 28 cells and SK 30 cells was not expressed. CONCLUSIONS: These results indicate that the identification of tyrosine protein kinase reeeptors and their inhibitor which affect differentiation and growth of a melanoma may provide an additional therapeutic option for treatment of melanoma.
