Specific expression of transmembrane protein 26 in retina and its association with primary open-angle glaucoma
10.3760/cma.j.cn115989-20200112-00021
- VernacularTitle:跨膜蛋白26在视网膜中的特异表达及其与原发性开角型青光眼的关系
- Author:
Yi YIN
1
;
Yao MAO
;
Zhenglin YANG
;
Lulin HUANG
Author Information
1. 电子科技大学医学院 四川省人民医院医学检验中心 人类疾病基因研究四川省重点实验室，成都 610072
- Keywords:
TMEM26 protein, human;
Tmem26 protein, mouse;
Protein transmembrane structure;
Retina;
Inner plexiform layer;
Outer plexiform layer;
Primary open-angle glau
- From:
Chinese Journal of Experimental Ophthalmology
2022;40(4):303-309
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To predict the transmembrane structure of transmembrane protein 26 (TMEM26), observe its expression in human retina and mouse retina, and investigate the relationship between it and primary open-angle glaucoma (POAG).Methods:The transmembrane structure of TMEM26 in human and mouse was obtained by inputting its amino acid sequences into the transmembrane protein structure prediction software, MemBrain.The expression and location of TMEM26 in human and mouse retinas were observed through frozen retinal sections stained with anti-TMEM26 antibody, which came from a human donor and five SPF-grade C57BL/6 mice.The possible function of TMEM26 gene and its influence on eyes were inferred on the basis of the specific expression of TMEM26 in retina.The single nucleotide polymorphism mutation of TMEM26 gene was searched in literature related to ocular diseases.The use and care of animals complied with the Regulations on the Management of Experimental Animals.This research protocol was approved by an Ethics Committee of Sichuan Provincial People's Hospital (No.2019-36). Results:Both human and mouse TMEM26 were eight transmembrane proteins with similar eight hydrophobic transmembrane domains, four hydrophilic cytoplasmic domains and five hydrophilic extracellular membrane domains.Small differences in the number of amino acid residues in the domains of TMEM26 were found.In both human and mouse retina, TMEM26 gene was only specifically expressed in the outer plexiform layer (OPL)and inner plexiform layer (IPL). TMEM26 was weakly associated with POAG in a published data. Conclusions:TMEM26 is a multi-pass transmembrane protein, mainly expressed in IPL and OPL of the retina. TMEM26 gene is weakly related to POAG.
