Expression and significance of long non-coding RNA metastasis associated lung adenocarcinoma transcript 1 in bronchopulmonary dysplasia of neonatal rats induced by hyperoxia
10.3760/cma.j.issn.1673-4912.2022.05.010
- VernacularTitle:长链非编码RNA转移相关肺腺癌转录本-1在高氧致新生鼠支气管肺发育不良中的表达及意义
- Author:
Lin FAN
1
;
Xianxian JIA
;
Bo WU
;
Wei XU
Author Information
1. 中国医科大学附属盛京医院儿内科，沈阳 110004
- Keywords:
Bronchopulmonary dysplasia;
Alveolar type Ⅱ epithelial cells;
Long non-coding RNA metastasis associated lung adenocarcinoma transcript 1;
Proliferation;
R
- From:
Chinese Pediatric Emergency Medicine
2022;29(5):368-372
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of long non-coding RNA metastasis associated lung adenocarcinoma transcript 1(lncRNA MALAT1) in bronchopulmonary dysplasia (BPD) of neonatal rats induced by hyperoxia and its effect on alveolar type 2 epithelial cells (AEC Ⅱ).Methods:The lung injury model of neonatal SD rats induced by hyperoxia(model group, n=50, inhaled oxygen concentration of 80%-85%) and the control group(inhaled air, n=50) were prepared.Lung tissue samples were taken and retained on days 1, 3, 7, 14 and 21, and the physiological and pathological changes of lung tissue were detected by paraffin-embedded sections and hematoxylin-eosin staining; The dynamic expression of lncRNA MALAT1 in lung tissue was detected by real-time fluorescent quantitative polymerase chain reaction; The dynamic expression of surfactant protein C(SPC) in lung tissue and AECⅡ was detected by Western blot.AECⅡ was extracted from lung tissue of normal newborn rats, and lncRNA MALAT1 was knocked down by siRNA.The cells were collected and Western blot as well as immunofluorescence were used to detect the changes of SPC. Results:The lung tissue of model group gradually became thickened with alveolar compartments, and the alveolar cavity was enlarged with the disappearance of alveolar spine and other pathological structural changes.Compared with the control group, there was no difference in the expression of lncRNA MALAT1 and SPC in the lung tissue from model group on days 1, 3( P>0.05), but the expression of lncRNA MALAT1 and SPC significantly increased on days 7, 14 and 21( P<0.05). When lncRNA MALAT1 was inhibited, SPC expression showed a decrease trend. Conclusion:Hyperoxia can lead to the stagnation of lung development in neonatal rats, and the structure and function of alveolar disorders are impaired.The expression of lncRNA MALAT1 is involved in the process of hyperoxia-induced BPD in neonatal rats.The increase of lncRNA MALAT1 may promote the proliferation of AECⅡ.
