Biomarkers of zinc deficiency in left-behind children under 1 year old in Zunyi area
10.3760/cma.j.cn101070-20190119-00041
- VernacularTitle:遵义地区1岁以下留守儿童锌缺乏症生物标志物研究
- Author:
Xiang ZHANG
1
;
Yu CHENG
;
Huiling SONG
;
Yunfeng XIANG
;
Hongjiao JIN
;
Qing LI
;
Xuqin WANG
;
Limei LUO
;
Yong LIN
;
Bo HUANG
Author Information
1. 遵义市第一人民医院（遵义医科大学第三附属医院）儿童重症医学科，遵义 563000
- Keywords:
Zinc deficiency;
Metabonomics;
Nuclear magnetic resonance technology;
Differential metabolites
- From:
Chinese Journal of Applied Clinical Pediatrics
2022;37(9):697-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the urine of normal healthy left-behind children under 1 year old and left-behind children with zinc deficiency under 1 year old in Zunyi area using hydrogen nuclear magnetic resonance ( 1HNMR), thus providing a new biomarker for the early diagnosis of zinc deficiency. Methods:From January to August 2018, a total of 40 normal healthy left-behind children under 1 year old in Zunyi area(healthy control group)[22 males and 18 females, average age of (7.78±3.62) months, average height of (65.01±2.67) cm and average body mass of (7.15±1.59) kg] and 40 age-matched left-behind children with zinc deficiency in the same region(zinc deficiency group)[19 males and 21 females, average age of (7.89±3.57) months, average height of (64.25±2.95) cm and average body mass of (7.02±1.68) kg] were included for a cross-sectional study by stratified sampling.The urine 1HNMR spectra of children in the 2 groups were measured, and the age, height, body mass and serum zinc content of children in the 2 groups were compared.The metabolites of the 2 groups were compared by metabono-mics technology combined with multivariate statistical analysis, and the differential metabolites of children with zinc deficiency were screened out. Results:There were no significant differences in age, height and body mass between the 2 groups (all P>0.05). The serum zinc level of healthy control group was significantly higher than that of zinc deficiency group [(54.3±3.06) mmol/L vs.(39.2±3.77) mmol/L, t=22.65, P<0.05]. Urine 1HNMR spectrogram results showed that compared with healthy controls, 4-hydroxyphenylpyruvic acid, phenyl acetyl glycine, and hippuric acid salt water were significantly lower in zinc deficiency group ( r=-0.620, -0.689, and -0.721, respectively, all | r|>0.602, all P<0.05). Conclusions:Zinc deficiency in left-behind children under 1 year old in Zunyi area is mainly manifested by decreased metabolites of 4-hydroxyphenylpyruvic acid, phenylacetyl glycine and horse-urate, suggesting metabolic disorder of intestinal flora.Differentially expressed metabolites have a potential application value in the early diagnosis of zinc deficiency.
