Diagnosis of 3 pediatric cases of scrub typhus without the eschar by metagenomics next-generation sequencing
10.3760/cma.j.cn101070-20200628-01071
- VernacularTitle:宏基因组二代测序技术协助诊断3例无焦痂儿童恙虫病
- Author:
Wei LIN
1
;
Beibei LIN
;
Zhenhai TANG
;
Yafeng LIANG
;
Guoquan PAN
Author Information
1. 温州医科大学附属第二医院、育英儿童医院儿童重症与急诊医学科，温州 325000
- Keywords:
Scrub typhus;
Eschar;
Pathogen;
Metagenomics next-generation sequencing;
Child
- From:
Chinese Journal of Applied Clinical Pediatrics
2022;37(3):210-213
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the pathogen of 3 pediatric cases of scrub typhus without the eschar using metagenomics next-generation sequencing (mNGS), and the clinical application value of mNGS.Methods:Clinical data of 3 pediatric cases of severe scrub typhus without the eschar in the Second Affiliated Hospital and Yuying Children′s Hospital of Wenzhou Medical University from June 2018 to October 2019 were analyzed retrospectively.Among them, 2 cases were 5-year-old males and 1 case was 6-year-old female.Peripheral blood samples of 3 children were detected via mNGS technology.Results:Through mNGS detection, all 3 children were diagnosed with Orientia tsutsugamushi infection and were not complicated with other pathogenic infections.Case 1 died, and case 2 and case 3 were cured.Conclusions:Early diagnosis of scrub typhus without the eschar is difficult.Clinical infectious diseases, especially complicated and critical infectious diseases are difficult to be diagnosed at an early stage, mNGS can provide fast and accurate pathogenic diagnosis support for precise treatment.
