Effects of miR-485-5p on cisplatin-resistant ovarian cancer cell lines via targeting EGFR
10.3760/cma.j.cn.115807-20200813-00246
- VernacularTitle:miR-485-5p靶向EGFR对卵巢癌顺铂耐药细胞系的影响
- Author:
Bing ZHU
1
;
Aiqin NIU
;
Kun WANG
Author Information
1. 商丘市第一人民医院妇科，商丘 476000
- Keywords:
Ovarian cancer;
Cisplatin resistance;
miR-485-5p;
EGFR
- From:
Chinese Journal of Endocrine Surgery
2022;16(2):201-206
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of miR-485-5p on cisplatin-resistant ovarian cancer cells and it’s mechanism.Methods:RT-qPCR was used to detect the expression of miR-485-5p in human normal ovarian epithelial cell line (IOSE-80) and ovarian cancer cell lines (A2780,SKOV3,OVCAR3,OVCA433) . Cisplatin (DDP) -resistant ovarian cancer cells were constructed and the expression of miR-485-5p and EGFR was detected. CCK8 assay was used to detect cell proliferation ability in each group. Cell apoptosis was measured by flow cytometry.Results:Compared with IOSE-80 cell, miR-485-5p expression was decreased in each ovarian cancer cell lines, while the expression of EGFR was increased (all P<0.05) .The expression of miR-485-5p in SKOV3/DDP cell lines was further decreased while EGFR expression was further increased than that in SKOV3 cells ( P<0.05) . Transfection of miR-485-5p mimic into SKOV3/DDP cells could inhibit cell proliferation and induce apoptosis, but the results were reversed when cells were transfected with miR-485-5p inhibitor (all P<0.05) . The proliferation was increased while apoptosis was decreased in EGFR transfected SKOV3/DDP cells, but this effects was partially offseted by miR-485-5pmimic. Conclusion:miR-485-5p participates in the regulation of cisplatin resistance of ovarian cancer cells, and overexpression of miR-485-5p can promote the chemosensitivity of ovarian cancer, which may be achieved through negative regulation of EGFR.
