Long non-coding RNA lnc-SLC2A12-10:1 derived from tumor-associated macrophage exosome aggravates the proliferation and invasion of breast cancer cells through miR-296-5p
10.3760/cma.j.cn.115807-20210908-00274
- VernacularTitle:肿瘤相关巨噬细胞外泌体lnc-SLC2A12-10:1调节miR-296-5p促进乳腺癌细胞增殖、侵袭
- Author:
Xin WANG
1
;
Yaoxia WANG
;
Zhimin JI
;
Jingdong LI
Author Information
1. 临沂市人民医院肿瘤科，临沂 276000
- Keywords:
Tumor-associated macrophages;
Exosomes;
Breast cancer;
lnc-SLC2A12-10:1
- From:
Chinese Journal of Endocrine Surgery
2022;16(2):155-159
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of the mechanism of lnc-SLC2A12-10:1 derived from tumor-associated macrophages (TAMs) exosomes on the proliferation and invasion of breast cancer (BC) cells.Methods:GEO microarray analysis was used to screen out the differentially expressed lnc-SLC2A12-10:1 in BC. qRT-PCR was performed to determine the expression level of lnc-SLC2A12-10:1 and miR-296-5p in tissue and cells. Then TAMs and exosomes were isolated. After, interfering the expression level of lnc-SLC2A12-10:1 in exosomes and miR-296-5p expression in cells, then cell proliferation and invasion were detected with the help of MTT and Transwell assays.Results:Compared with adjacent tissues, lnc-SLC2A12-10:1 was significantly up-regulated in BC tissues ( t=7.09, P<0.001) . Compared with normal breast cells, the expression of lnc-SLC2A12-10:1 in T47D and MDA-MB-468 cells was significantly up-regulated ( t=9.90, P<0.001) ( t=12.18, P<0.001) . lnc-SLC2A12-10:1 could act as a ceRNA of miR-296-5p. Knockdown of lnc-SLC2A12-10:1 inhibited BC cell proliferation and invasion, miR-296-5p inhibitor promoted BC cell proliferation and invasion, but this effect could be partially rescued by si--lnc-SLC2A12-10:1-Exo (all P<0.05) . Conclusion:lnc-SLC2A12-10:1 derived from TAMs exosomes promotes BC cell proliferation, invasion and thus advance BC progression.
