The role of Circular RNA circ_0000253 in diagnosis and prognosis of osteosarcoma
10.3760/cma.j.cn.115807-20210401-00107
- VernacularTitle:探讨Circ_0000253在骨肉瘤中的诊断价值
- Author:
Hua LI
1
;
Li GUO
;
Gangqiang ZHANG
;
Li LI
;
Bin HAN
Author Information
1. 安阳市中医院肿瘤科，安阳 455000
- Keywords:
Osteosarcoma;
Circular RNA
- From:
Chinese Journal of Endocrine Surgery
2022;16(1):113-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the value of circ_0000253 as a biomarker for (osteosarcoma, OS) diagnosis and prognosis.Methods:GEO database was used to screen the differentially expressed circRNAs in OS, microRNAs (miRNAs) with binding sites of circRNA were predicted to further construct the ceRNA network, and GO and KEGG analysis were used to screen the target genes. qRT-PCR was used to detect the expression of circ_0000253, miR-578 and TGF β2 in cancer and paracancerous tissue in OS patients, as well as OS cells and normal osteoblasts. The diagnostic value of circ_0000253 for OS and its impact on prognosis were analyzed, and the relationship between circ_0000253 expression level and clinicopathological parameters of patients was discussed. Further the expression of circ_0000253 and miR-578 in cells was intervened, apoptosis was detected by flow cytometry and cell proliferation was detected by MTT assay. Results:The expression of circ_0000253 in OS tissue was higher than that in paracancerous tissue ( t=11.17, P<0.001) . The expression of circ_0000253 was significantly correlated with Enneking stage, histopathology grade and distant metastasis (all P<0.05) . Circ_0000253 can be used as an effective diagnostic index of OS (AUC=0.84, P<0.001) and the prognosis of patients with high expression of circ_0000253 is poor. Circ_0000253 may regulate OS through miR-578/TGF β2 network. Knockdown of circ_0000253 reduced cell proliferation and induced apoptosis, which could be partially saved by miR-578 inhibitor (all P<0 05) . Conclusion:circ_0000253 may serve as a new biomarker for OS diagnosis and prognosis, which has a positive meaning for targeted therapy of OS.
