Sevoflurane regulates cell activity and apoptosis of breast cancer via Circ_001589/miR-588
10.3760/cma.j.cn.115807-20211019-00318
- VernacularTitle:七氟醚通过Circ_001589/miR-588调控乳腺癌细胞活力与凋亡
- Author:
Chunyu LYU
1
;
Liguo GONG
;
Qiang CHEN
;
Chen LI
;
Chenghai WANG
Author Information
1. 烟台市烟台山医院麻醉科 264000
- Keywords:
Circ_001589;
Breast cancer;
Sevoflurane;
miR-588
- From:
Chinese Journal of Endocrine Surgery
2021;15(6):651-656
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects and molecular mechanism of circ_001598 on biological behavior of breast cancer (BC) cells.Methods:Sevoflurane in different concentrations were used to treat BC cells and the cell activity and apoptosis were detected. qRT-PCR was used to determine the relative expression of Circ_001598 and miR-588 in BC tissue and cells. The effects of Sevoflurane on Circ_001598, miR-588 expression was detected. Dual luciferase reporter gene assay was used to detect the relationship between Circ_001598 and miR-588. The expression of Circ_001598, miR-588 in BC cells was intervened, then cell activity and apoptosis level was detected by using MTT and flow cytometry individually.Results:Sevoflurane inhibited cell activity of BC cells, and promoted cell apoptosis. Circ_001598 was increased in BC tissue and cells, but Sevoflurane could down-regulate the expression of Circ_001598 (all P<0.05) . Overexpression of Circ_001589 partially saved the effects of Sevoflurane on cell viability and apoptosis. Circ_001598 negatively regulated miR-588 in BC cells. miR-588 expression was down-regulated in BC tissue and cells, but Sevoflurane up-regulated the expression level of miR-588 in BC cells (all P<0.05) . miR-588 transfection partially offseted the effects of Circ_001598 on Sevoflurane induced BC cells apoptosis. Conclusion:Sevoflurane affects BC cell viability and apoptosis via regulating Circ_001589/miR-588.
