Effects of ultrasound microbubble-mediated G3BP1 transfection on proliferation and migration of human hepatocarcinoma HepG2 Cells
10.3760/cma.j.cn.115807-20210818-00252
- VernacularTitle:超声微泡介导G3BP1转染对人肝癌HepG2细胞增殖、迁移的影响
- Author:
Wenfan HUANG
1
;
Wen ZHOU
;
Yilan ZHANG
;
Yan HUANG
Author Information
1. 杭州市余杭区第一人民医院超声科 311100
- Keywords:
RasGAP SH3-binding protein 1;
Ultrasound-targeted microbubble destruction;
Liver cancer;
HepG2 cells;
Proliferation;
Migration
- From:
Chinese Journal of Endocrine Surgery
2021;15(6):622-626
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of ultrasound microbubble-mediated RasGAP SH3-binding protein 1 (G3BP1) transfection on the proliferation and migration of human liver cancer HepG2 cells.Methods:HepG2 cells were treated with ultrasound targeted microbubble destruction (UTMD) technology-mediated si-G3BP1. The expression of G3BP1 in HepG2 cell lines was detected by Western blot, and the silencing efficiency was analyzed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot. HepG2 cell proliferation and migration were analyzed by flow cytometry, methyl thiazolyl tetrazolium (MTT) , EdU staining, colony formation experiment, wound healing experiment, Transwell experiment and Western blot.Results:After silencing G3BP1 in HepG2 cells, its mRNA and protein levels were significantly reduced (1.01±0.03 vs 0.27±0.03, 1.02±0.01 vs 0.33±0.04) ; UTMD-mediated si-G3BP1 could significantly reduce the proliferation rate (31.49±3.09 vs 12.51±1.02) , proliferation activity (1.20±0.13 vs 0.46±0.31) , EdU-positive cell rate (99.23±1.01 vs 36.75±4.03) , colony formation rate (96.45±1.21 vs 32.67±2.62) , scratch healing rate (97.58±1.04 vs 42.33±2.56) , migration rate (94.28±2.33 vs 39.36±2.51) and Ki67, Cyclin D1, Vimentin protein levels, increase E-cadherin protein levels.Conclusion:UTMD-mediated si-G3BP1 can inhibit the proliferation and migration of human liver cancer HepG2 cells.
