Analysis of the hypoglycemic effect of sleeve gastrectomy on obese and diabetic rats and exploration of its mechanism
10.3760/cma.j.cn.115807-20200315-00081
- VernacularTitle:袖状胃切除术对肥胖合并糖尿病大鼠的降糖效果分析及机制探索
- Author:
Haojun YANG
1
;
Yuwen JIAO
;
Hanyang LIU
;
Jun QIAN
Author Information
1. 南京医科大学附属常州市第二人民医院胃肠外科 213000
- Keywords:
Sleeve gastrectomy;
Bile acid;
Type 2 diabetic;
Bile acid G protein coupled receptor (TGR5)
- From:
Chinese Journal of Endocrine Surgery
2021;15(6):594-597
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of sleeve gastrectomy on blood glucose in obese rats with diabetes mellitus.Methods:Thirty-two 12 week old Goto Kakizaki (GK) diabetic rats were randomly divided into 4 groups: sham operation group (A) , sleeve stomach operation group (B) , sleeve stomach operation+external bile drainage group (C) and sleeve stomach operation + external bile drainage + oleanolic acid group (D) . The changes of fasting blood glucose and blood bile acid were compared before and after operation. The expression level of GLP-1 in serum of each group was detected by ELISA, and the difference of protein expression of bile acid G protein coupled receptor (TGR5) was detected by Western blot.Results:The blood glucose level of group A had no significant change after operation. But blood glucose level in group B and group D was significantly lower than that before operation. Blood glucose in group C was lower than that before operation, but there was no significant difference in comparison. Serum total bile acid level in group A had no significant difference before and after operation. Bile acid level in group B was significantly higher than that before operation, while bile acid level in group C and group D was significantly lower than that before operation. There was no significant difference in the level of serum GLP-1 in group A before and after operation. The level of serum GLP-1 in group B and group D was significantly higher than that before operation. The level of serum GLP-1 in group C was lower than that before operation, but there was no significant difference. The expression of TGR5 protein in terminal ileum in group B and group D was higher than that in group A, but the expression in group C was similar with that in group A.Conclusions:Sleeve gastrectomy has definite hypoglycemic effect on T2DM rats. Bile acid and its related receptor TGR5 should play an important role in the mechanism of sleeve gastrectomy in treatment of diabetes.
