Analysis of the diversity and structural changes of intestinal flora in patients with sepsis
10.3760/cma.j.issn.1671-0282.2022.04.015
- VernacularTitle:脓毒症患者肠道菌群多样性和结构变化分析
- Author:
Lan GUAN
1
;
Heng ZHOU
;
Cong WANG
;
Zhiwei LIU
;
Bin ZHAO
Author Information
1. 北京积水潭医院急诊科，北京 100035
- Keywords:
Sepsis;
Intestinal flora;
Fecal;
16S rRNA
- From:
Chinese Journal of Emergency Medicine
2022;31(4):514-519
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the diversity and structural changes of intestinal flora in patients with sepsis.Methods:A total of 55 patients were enrolled who visited the Emergency Department of several Grade III-A hospitals from November 2015 to March 2016. Among them, 25 patients with sepsis metthe diagnostic criteria of Sepsis 3.0 and 30 patients had no sepsis. The intestinal flora from all fecal samples was sequenced by 16S rRNA sequencing technology. The diversity and structure changes of the flora were analyzed using Uparse, Qiime, R and LEfSe software.Results:There was no significant difference in sex, age, chronic underlying diseases, and the diversity of intestinal flora between the sepsis and non-sepsis groups (all P>0.05). The structure of intestinal flora was significant different between the two groups ( P<0.05). Abundances of Negativicutes, Selenomonadales, Veillonellaceae, Lachnospiraceae, Faecalibacterium, Hafnia, Lachnoclostridium, Blautia and Ruminococcus in intestinal flora from patients with sepsis were significantly reduced, but those of Bacilli, Coriobacteriia, Lactobacillales, Coriobacteriales, Clostridiaceae, Coriobacteriaceae, Clostridium_sensu_stricto, Collinsella and Collinsella_aerofaciens were significantly increased. Conclusions:There is no change in the diversity of intestinal flora between the sepsis and non-sepsis groups. There are structural changes of intestinal flora in patients with sepsis.
