Characteristic analysis of Panton-Valentine leukocidin in children with invasive staphylococcus infection
10.3760/cma.j.issn.1671-0282.2022.04.009
- VernacularTitle:儿童金黄色葡萄球菌感染中杀白细胞素的特征
- Author:
Rubo LI
1
;
Fangfang DONG
;
Yan XU
;
Chen SUN
;
Xin YANG
;
Yingchao LIU
;
Lijuan WANG
;
Suyun QIAN
;
Kaihu YAO
Author Information
1. 国家儿童医学中心，首都医科大学附属北京儿童医院重症医学科, 北京 100045
- Keywords:
Staphylococcus aureus;
Child;
Staphylococcal infections;
Panton-Valentine leukocidin;
Prognosis;
Molecular characteristics;
Virulence;
Antimicrobial suscepti
- From:
Chinese Journal of Emergency Medicine
2022;31(4):477-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the relationship between the Panton-Valentine leukocidin (pvl) strain and clinical characteristics, and to describe the molecular biological characteristics of invasive Staphylococcus aureus ( S. aureus) infected clinical isolates. Methods:The isolates of S. aureus caused by invasive infection were collected in Beijing Children's Hospital Affiliated to Capital Medical University from January 2016 to December 2019, and the clinical data of the corresponding children were collected retrospectively using electronic medical records. Multilocus sequence typing, spa typing and pvl gene were analyzed using the PCR. In addition, the minimum inhibitory concentrations (MIC) of antibiotics of all isolates were detected by the micro-broth dilution method, and the isolates were divided into the pvl+ and pvl- groups according to whether or not the S. aureus isolates carried pvl. The t test and the Mann-Whitney U test were used to compare the clinical symptoms between the pvl+ and pvl- groups. Chi-square test was used to compare the drug susceptibility between the two isolates. Results:A total of 127 cases of invasive S. aureus infection were collected during the study period. The white blood cell count, neutrophil count, and C-reaction protein level in the pvl+ group were significantly higher than those in the pvl- group ( P=0.001, P=0.001, P=0.005). The rate of pvl carrier was 44.9%. Among 57 pvl+ pathogenic strains, 64.9% (37/57) were MRSA. The multidrug resistance rate of pvl- isolates was higher than that of pvl+ isolates (70% vs. 49.12%, P=0.02). Conclusions:In invasive S. aureus infection, pvl+ strain is associated with elevated inflammatory markers in children. the positive rate of pvl is higher in clinical isolates, and the multidrug resistance rate of pvl- S. aureus is higher.
