Gabapentin alleviates myocardial ischemia-reperfusion injury through the PI3K-AKT signaling pathway
10.3760/cma.j.issn.1671-0282.2022.03.015
- VernacularTitle:加巴喷丁通过PI3K-AKT信号通路减轻大鼠心肌缺血—再灌注损伤
- Author:
Bin WU
1
;
Yahong CAO
;
Rui LI
Author Information
1. 安徽医科大学第二附属医院麻醉与围术期医学科，合肥 230601
- Keywords:
Gabapentin;
Myocardial ischemia/reperfusion injury;
PI3K/AKT pathway;
arrhythmia;
Myocardial infarction
- From:
Chinese Journal of Emergency Medicine
2022;31(3):344-348
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of gabapentin on myocardial ischemia-reperfusion injury and its mechanism.Methods:Sixty male clean SD rats, aged 10 weeks and weighing 250 g~300 g, were divided into 5 groups ( n=12) with 12 rats in each group by random number table method: Sham group, myocardial ischemia reperfusion group (group I/R), gabapentin group (group Gap), LY294002 group (group LY) and gabapentin +LY294002 group (group Gap +LY). The model of myocardial ischemia reperfusion injury was established by ligation of the left anterior descending coronary artery for 30 min and reperfusion for 120 min. Heart rate (HR), mean arterial pressure (MAP) and the rate pressure product (RPP) were recorded at baseline before ischemia (T 0) for 30 min (T 1) and reperfusion for 120 min (T 2) to evaluate hemodynamic changes during ischemia and reperfusion; The frequency of PVCs and VT/VF were recorded to evaluate the occurrence of arrhythmias during ischemia/reperfusion. TTC staining was used to detect myocardial infarction area. And the protein expression levels of PI3K and Akt in myocardial tissue were detected by Western blotting. Results:Compared with group I/R, the myocardial infarction area, PVCs and VT/VF times, and the protein expression levels of PI3K and p-Akt were significantly increased in group Gap ( P<0.05). Compared with group Gap, the area of myocardial infarction, the number of PVCs and VT/VF, and the protein expression of PI3K and p-Akt were significantly decreased in the group Gap +LY ( P<0.05). Conclusions:Gabapentin can alleviate myocardial ischemia-reperfusion injury, and its mechanism is related to the activation of PI3K-AKT signaling pathway.
