Metabonomics research of gabex mesylate on septic-induced acute lung injury in rats
10.3760/cma.j.issn.1671-0282.2022.02.010
- VernacularTitle:甲磺酸加贝酯改善脓毒症急性肺损伤大鼠的代谢组学研究
- Author:
Ning WANG
1
;
Xianfei DING
;
Yuqing CUI
;
Yali SUN
;
Heng SONG
;
Junyi SUN
;
Gaofei SONG
;
Yangyang YUAN
;
Tongwen SUN
Author Information
1. 郑州大学第一附属医院综合ICU 河南省重症医学重点实验室 郑州市脓毒症重点实验室 河南省重症医学工程研究中心，郑州 450052
- Keywords:
Gabexate mesylate;
Sepsis;
CLP;
Acute lung injury;
Metabonomics
- From:
Chinese Journal of Emergency Medicine
2022;31(2):191-196
- CountryChina
- Language:Chinese
-
Abstract:
Objectve:To study the effect of gabexate mesylate (GM) on acute lung injury (ALI) in septic rats based on metabonomics.Methods:Fifty-seven SD rats were randomly(random number) divided into three groups: sham operation group (SC group), cecal ligation puncture induced septic ALI group (CLP group), and intraperitoneal administration of GM at 1 h after CLP (CLP-GM group). Twenty-four h after the experiment, the survival of rats in the SC, CLP and CLP-GM groups was observed, the lung tissue was collected for HE staining to observe the pathological changes, and the plasma was collected for metabonomics detection to analyze the characteristics of metabolites.Results:Compared with the SC group, the infiltration of inflammatory cells in the lung tissue of rats in the CLP groupincreased significantly, and the metabolic profile of plasma changed significantly. However, the pathological and metabonomic characteristics of the CLP-GM group showed that the above changes were reversed after the application of GM. Twelve major differential metabolites were found in plasma. The metabolic pathways involved in the disorder included biosynthesis of phenylalanine, tyrosine and tryptophan, phenylalanine metabolism and sphingolipid metabolism.Conclusions:GM may improve septic ALI by regulating amino acid metabolism, sphingolipid metabolism and other metabolic pathways.
