Analysis of the spectrum-effect relationship of different processed products of Epimedii folium on cytotoxicity
10.3760/cma.j.cn115398-20211013-00113
- VernacularTitle:淫羊藿不同炮制品对细胞毒性影响的谱效关系分析
- Author:
Yue ZHANG
1
;
Zimin YUAN
;
Ye NIU
;
Mingyue YUAN
;
Jing WANG
Author Information
1. 辽宁中医药大学药学院，大连 116600
- Keywords:
Epimedii folium;
Processing (TCD);
Toxic actions;
Grey correlation analysis;
Spectrum effect relationship
- From:
International Journal of Traditional Chinese Medicine
2022;44(5):547-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of different processed products of Epimedii Folium on cytotoxicity and the material basis of toxicity. Methods:By using the SRB method to investigate the effects of different processed products of Epimedii Folium on the proliferation of HaCaT cells; and based on the grey correlation analysis method to establish the data spectrum effect relationship of HPLC fingerprint spectrum-toxicity so as to determine the toxic components and processing methods. Results:The value of cytotoxicity IC 50 of different processed products of Epimedii Folium is as follow: vinegar fried> oil fried > original > single fried > salt fried > wine fried. Among the 12 characteristic chromatographic peaks, Peak No.3 (magnoflorine, correlation value: 0.870) and Peak No.6 (epimedin C, correlation value: 0.851) are highly correlated with the toxicity value. Conclusions:Both vinegar fried and oil fried Epimedii Folium have the effect of reducing toxicity. Magnoflorine and epimedin C may be the main toxic components in Epimedii Folium. The study provides scientific basis for the research on the process optimization of Epimedii Folium concocting to reduce toxicity.
