Determination of Calycosin-7-O-β-D-Glucopyranoside and Lobetyolin in Danqi Xinmaikang boiled powders and pieces by quantitative analysis of multi-components by single maker
10.3760/cma.j.cn115398-20210316-00186
- VernacularTitle:一测多评法测定丹芪心脉康煮散及饮片中毛蕊异黄酮葡萄糖苷和党参炔苷含量
- Author:
Zilong ZHANG
1
;
Luna XIE
;
Shunyu LI
;
Yu LIU
;
Jiarui LI
;
Qi LIANG
Author Information
1. 深圳市宝安中医院（集团）药学部，深圳 518000
- Keywords:
Assay (TCD);
Quantitative analysis of multi-components by single marker;
Calycosin-7-O-β-D-Glucopyranoside;
Lobetyolin;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2022;44(3):309-313
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a quality evaluation method for the simultaneous determination of Calycosin-7-O-β-D-Glucopyranoside and Lobetyolin in Danqi Xinmaikang boiled powders and pieces.Methods:Quantitative analysis of multi-components was performed to determine contents of Calycosin-7-O-β-D-Glucopyranoside and Lobetyolin with Calycosin-7-O-β-D-Glucopyranoside as the reference substance by single-maker (QAMS). The chromatogram conditions were established, with C18 column as solid phase, acetonitrile-water as flowing phase, 268 nm as detecting wavelength, 1.0 ml/min as flowing rate, 30 ℃ as column temperature, and 10 μl as injection volume.Results:The relative correction factor between Calycosin-7-O-β-D-Glucopyranoside and Lobetyolin was 1.14. There was no significant difference of measured values between the external standard method and QAMS ( P>0.05). With Calycosin-7-O-β-D-Glucopyranoside retention time of 1.00, the relative retention time of Lobetyolin was 1.51 and RSD was less than 5%. Conclusion:It is feasible and accurate to evaluate the quality of Danqi Xinmaikang boiled powders and pieces by QAMS.
