Mechanism of silencing miR-4320 expression in inhibiting proliferation and migration of gastric cancer cells
10.3760/cma.j.issn115396-20220414-00123
- VernacularTitle:沉默miR-4320抑制胃癌细胞增殖和迁移的作用机制研究
- Author:
Shouyuan TANG
1
;
Jinping JIANG
;
Zhongzhong ZHU
;
Haiping LUO
;
Weijie ZHANG
;
Guoyu LAN
Author Information
1. 鄂东医疗集团黄石市中心医院（湖北理工学院附属医院）胃肠肛肠外科，黄石 435000
- Keywords:
Stomach neoplasms;
Cell proliferation;
Cell migration assays;
miR-4320;
Suppressor of cytokine signaling 1
- From:
International Journal of Surgery
2022;49(5):306-309,C1
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect and mechanism of microRNA (miRNA)-4320 on the proliferation and migration of gastric cancer MGC803 cells.Methods:Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-4320 in four gastric cancer cell lines(MGC803, HS-746T, SGC7901, BGC823) MGC803 cells were infected with recombinant lentivirus carrying miR-4320 interference fragments or blank lentivirus, and set as si-miR-4320 group and NC group. Thiazole blue colorimetry and Transwell small box experiment were used to detect the proliferation and migration of MGC803 cells after miR-4320 was down-regulated. The bioinformatics software RNAhybrid was used to predict the target gene of miR-4320. The targeting relationship between miR-4320 and target gene was verified by dual-luciferase reporter gene experiment. qRT-PCR and Western blot were used to detect the expression of miR-4320 target gene. Measurement data were expressed as mean ± standard deviation ( ± s), and t-test or one-way ANOVA was used for comparison between groups. Results:The expression of miR-4320 in the four gastric cancer cell lines was significantly higher than that of normal gastric mucosal epithelial cells ( P<0.01). The expression of miR-4320 in MGC803 cells in the NC group and the si-miR-4320 group were 8.19±1.00 and 1.09±0.31, respectively. The miR-4320 interference fragment significantly reduced the expression of miR-4320 ( P<0.01). The absorbance of MGC803 cells in the si-miR-4320 group was significantly lower than that of the NC group ( P<0.05), and the migration ability was significantly lower than that of the NC group ( P<0.01). Suppressor of cytokine signaling1 ( SOCSI) is the target gene of miR-4320. Compared with the NC group, the SOCS1 gene expression in the si-miR-4320 group was significantly up-regulated ( P<0.01). Conclusions:The expression of miR-4320 is increased in gastric cancer cell lines. Down-regulating the expression of miR-4320 can inhibit the proliferation and migration of gastric cancer MGC803 cells by inducing the expression of SOCS1 gene.
