Establishment of a clinical grade human umbilical cord mesenchymal stem cells bank
10.3760/cma.j.cn121382-20210515-00606
- VernacularTitle:临床级人脐带间充质干细胞资源库的构建
- Author:
Qinghui ZHAO
1
;
Zhihui BAI
;
Wenwen JIA
;
Hongming TANG
;
Zhongmin LIU
Author Information
1. 同济大学附属东方医院干细胞转化医学产业基地，国家干细胞转化资源库，上海干细胞临床转化研究院，上海市干细胞临床诊疗工程研究中心，上海 200123
- Keywords:
Human umbilical cord mesenchymal stem cells;
Stem cell bank;
Clinical research;
Clinical application
- From:
International Journal of Biomedical Engineering
2021;44(6):454-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a three-level clinical grade human umbilical cord mesenchymal stem cells (hUC-MSCs) bank, including seed cell bank (SCB), master cell bank (MCB) and working cell bank (WCB), and provide hUC-MSCs with controllable quality for clinical research and application.Methods:247 human umbilical cord tissues were isolated, cultured, amplified, subcultured and frozen in GMP laboratory, and the biological characteristics, safety and stability of hUC-MSCs were tested in accordance with the requirements of relevant quality management control specifications.Results:247 strains of hUC-MSCs were isolated and prepared. The prepared hUC-MSCs have good purity and homogeneity without tumorigenicity, show good differentiation ability in biological efficacy, and have strong immunosuppressive effect in the process of co-culture with immune cells. These cells have passed the quality check of National Institute for Food and Drug Control. In this study, a three-level hUC-MSCs bank was established, and it was included into the National Stem Cell Translational Resource Center.Conclusions:A three-level clinical hUC-MSCs bank was successfully established and preliminarily applied to clinical research, which effectively promoted the standardized development of clinical stem cell resource bank and the clinical transformation and application of stem cells in China.
