Effects of siRNA mediated interference with URG11 expression on the biological function of osteosarcoma cell line MG63 and Wnt/β-catenin signalling pathway
10.3760/cma.j.cn121382-20210602-00602
- VernacularTitle:siRNA干扰URG11表达对骨肉瘤细胞系MG63的生物学功能和Wnt/β-catenin信号通路的影响
- Author:
Chenggong DONG
1
;
Wei ZHANG
;
Ying LIU
;
Ming ZHANG
Author Information
1. 烟台市烟台山医院病理科 264002
- Keywords:
Osteosarcoma;
URG11;
Cell proliferation;
Cell invasion;
Cell apoptosis;
Wnt/β-catenin signaling pathway
- From:
International Journal of Biomedical Engineering
2021;44(6):430-435
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of siRNA mediated interference with URG11 expression on the biological function of osteosarcoma cell line MG63 and Wnt/β-catenin signalling pathway.Methods:MG63 cells were divided into control group (un-transfected), NC-siRNA group (transfected with non-specific NC-siRNA) and URG11-siRNA group (transfected with URG11-siRNA). The expression of URG11 was detected by RT-PCR. The proliferation, invasion and apoptosis of MG63 cells were determined by CCK-8 assay, Transwell assay and flow cytometry, respectively. The protein expressions of URG11 and molecules in the Wnt/β-catenin signalling pathway (β-catenin, c-Myc, Cyclin D1, MMP-2 and Survivin) were detected by Western blot method.Results:Compared with the control group, URG11-siRNA transfection significantly reduced the mRNA and protein expressions of URG11 as well as the protein expressions of URG11, β-catenin, c-Myc, Cyclin D1, MMP-2 and Survivin in MG63 cells, which was accompanied by the decreased proliferation, inhibited invasion and enhanced apoptosis of MG63 cells (all P<0.05). There were no significant changes in the biological function and Wnt/β-catenin signalling pathway in the MG63 cells after NC-siRNA transfection. Conclusions:Interfering the expression of URG11 can inhibit the proliferation and invasion of MG63 cells and promote cell apoptosis, and the mechanism may be related to the inhibition of the activation of Wnt/β-catenin signaling pathway.
