Rutin attenuates early brain injury after subarachnoid hemorrhage in rats by protecting blood-brain barrier
10.3760/cma.j.issn.1673-4165.2021.12.007
- VernacularTitle:芦丁通过保护血脑屏障减轻大鼠蛛网膜下腔出血后早期脑损伤
- Author:
Yuwei HAN
1
;
Chenchen WANG
;
Guobiao LIANG
;
Xiaoming LI
Author Information
1. 北部战区总医院神经外科，沈阳 110016
- Keywords:
Rutin;
Subarachnoid hemorrhage;
Blood-brain barrier;
Treatment outcome;
Neuroprotection;
Rats
- From:
International Journal of Cerebrovascular Diseases
2021;29(12):914-919
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of rutin on blood-brain barrier in early brain injury after subarachnoid hemorrhage (SAH) in rats.Methods:A rat model of SAH was induced by puncturing the internal carotid artery. The rats were divided into a sham operation group, a model group and a rutin (50 mg/kg) group. Twenty-four hours after modeling, SAH score and neurological deficit score were conducted, and brain water content and Evans blue extravasation rate were detected in each group. Western blot analysis was used to detect the expression of claudin-5, occludin and zonula occluden (ZO)-1. TUNEL staining was used to detect neuronal apoptosis.Results:Compared with the sham operation group, the SAH score increased, the neurological deficit score decreased, and the brain water content and Evans blue extravasation increased in the model group. Rutin could significantly reduce the SAH score, increase neurological deficit score, and reduce brain water content and Evans blue exudation (all P<0.01). Western blot analysis showed that the expression of claudin-5, occludin and ZO-1 protein decreased in the model group, and the expression of claudin-5, occludin and ZO-1 protein increased significantly in the rutin group ( P<0.01). In addition, the number of TUNEL positive cells induced by SAH in the rutin group decreased. Conclusion:Rutin can play a protective role in early brain injury after SAH, and its mechanism may be associated with protecting the integrity of blood-brain barrier.
