Myocardial protective effect of Yangxin dingji capsule on diabetic cardiomyopathy model golden hamsters
- VernacularTitle:养心定悸胶囊对糖尿病心肌病模型金黄地鼠的心肌保护作用
- Author:
Yi ZHONG
1
;
Gang LIU
2
;
Guoping MA
3
;
Baozhu DING
1
;
Xiaoxia LI
1
;
Haoying LIU
1
;
Wenjie LIANG
1
Author Information
1. College of Integrated Chinese and Western Medicine，Hebei University of Chinese Medicine，Shijiazhuang 050200，China
2. First Dept. of Cardiology Medicine，the First Hospital Hebei Medical University，Shijiangzhuang 050000，China
3. Hebei Higher Education Institute Applied Technology Research Center on TCM Formula Preparation，Shijiazhuang 050200，China
- Publication Type:Journal Article
- Keywords:
Yangxin dingji capsule;
diabetic cardiomyopat hy;
NLRP3/caspase-1/GSDMD signaling pathway;
cardiomyocyte
- From:
China Pharmacy
2022;33(13):1573-1580
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore t he protective mechanism of Yangxin dingji capsules on the cardiomyocytes of diabetic cardiomyopathy（DCM）model golden hamsters. METHODS In this study ，golden hamsters were divided into control group （n＝ 10，no modeling ，no drug administration ），model group （n＝9，modeling，no drug administration ），TCM high-dose group [ n＝8， modeling，Yangxin dingji capsules 2 g/（kg·d）]，TCM low-dose group [ n＝8，modeling，Yangxin dingji capsules 1 g/（kg·d）] and empagliflozin group [ n＝9，positive control ，modeling，10 mg/（kg·d）]. All the golden hamsters were gavaged continuously for 8 weeks. The general conditions of golden hamsters were observed during the experiment. Blood glucose ，total cholesterol （TC）and creatine kinase MB （CK-MB），ejection fraction （EF），fractional shortening （FS），interleukin 1β（IL-1β）and transforming growth factor β1（TGF-β1）were detected ；the histopathological changes of myocardium were observed. mRNA and protein expression of nucleotide-binding oligomerization domain-like receptor protein 3（NLRP3），caspase-1，aspirin D （GSDMD），nuclear factor κB （NF-κB）and IL- 1β were detected and observed；DNA damage in myocardial was detected. RESULTS Compared with control group，the blood glucose ，TC，CK-MB，serum IL- 1β，TGF-β1 levels，the mRNA expressions and positive protein expression of NLRP 3，caspase-1，GSDMD，NF-κ B and IL-1 β and protein expression of GSDMD in golden hamsters were significantly increased in model group （P＜0.05 or P＜0.01） EF and FS were significantly decreased （P＜0.01）；the fibers of myocardial cells was disordered ， and the blue-stained collagen fibers between the myocardium increased ； DNA damaged positive cells in myocardial tiss ue of gold hamsters increased significantly. Compared with model group，the above indexes of administration groups were reversed to varying degrees ；the gap of myocardial cells were clear ，and the fibers disorder was improved ；the DNA damaged positive cells in the myocardial tissue were reduced to varying degrees. CONCLUSIONS Yangxin dingji capsule can inhibit the cardiomyocyte pyroptosis and relieve the inflammatory injury of DCM in DCM model golden hamsters by regulating the NLRP 3/caspase-1/GSDMD signaling pathway ，so as to protect the cardiomyocytes.
