Establishment of a platelet production model by bone marrow cavity transplantation of mouse primary megakaryocytes.
10.3760/cma.j.issn.0253-2727.2022.04.002
- Author:
Bai Ming HUANG
1
;
Xiao Yuan CHEN
1
;
Mei Juan XIA
1
;
Lin ZHENG
1
;
Cui Cui LIU
1
;
Jing Jing ZHAO
1
;
Pei SU
1
;
Hong Tao WANG
1
;
Jia Xi ZHOU
1
Author Information
1. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
- Publication Type:Journal Article
- Keywords:
Intramedullary injection;
Megakaryocyte;
Platelet
- MeSH:
Animals;
Blood Platelets;
Bone Marrow;
Bone Marrow Cells;
Bone Marrow Transplantation;
Humans;
Megakaryocytes/metabolism*;
Mice
- From:
Chinese Journal of Hematology
2022;43(4):272-278
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an intramedullary transplantation model of primary megakaryocytes to evaluate the platelet-producing capacity of megakaryocytes and explore the underlying regulatory mechanisms. Methods: Donor megakaryocytes from GFP-transgenic mice bone marrow were enriched by magnetic beads. The platelet-producing model was established by intramedullary injection to recipient mice that underwent half-lethal dose irradiation 1 week in advance. Donor-derived megakaryocytes and platelets were detected by immunofluorescence staining and flow cytometry. Results: The proportion of megakaryocytes in the enriched sample for transplantation was 40 to 50 times higher than that in conventional bone marrow. After intramedullary transplantation, donor-derived megakaryocytes successfully implanted in the medullary cavity of the recipient and produce platelets, which showed similar expression of surface markers and morphology to recipient-derived platelets. Conclusion: We successfully established an in vivo platelet-producing model of primary megakaryocytes using magnetic-bead enrichment and intramedullary injection, which objectively reflects the platelet-producing capacity of megakaryocytes in the bone marrow.
