The expression of ITGB2 in renal cell carcinoma and its effect on malignant biological behaviors of ACHN cells
DOI：10.3872/j.issn.1007-385x.2022.05.008
- VernacularTitle:ITGB2在肾细胞癌组织中的表达及其对ACHN细胞恶性生物学行为的影响
- Author:
WANG Qi
1
;
FAN Bo△
1
;
LIU Bin
1
;
MA Yongliang
1
;
REN Zongtao
1
;
ZHANG Aili
1
Author Information
1. Department of Urology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China
- Publication Type:Journal Article
- Keywords:
整合素β2；肾细胞癌；ACHN细胞；增殖；迁移；侵袭；细胞周期
- From:
Chinese Journal of Cancer Biotherapy
2022;29(5):442-448
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective：To investigate the expression of ITGB2 (integrinβ2) in renal cell carcinoma (RCC) tissues and cells (ACHN cells) and its effects on the proliferation, migration, invasion and cell cycle of ACHN cells. Methods: The expression level of ITGB2 in RCC tissues and para-cancerous tissues was analyzed by GEPIA database. The tissue samples of 66 RCC patients retained in the biological specimen bank of the Fourth Hospital of Hebei Medical University from 2016 to 2020 were selected for this study. The expression level of ITGB2 in 66 cases of RCC tissues and para-cancerous tissues was detected by immunohistochemical SP and qPCR, and the relationship between ITGB2 and clinical parameters was analyzed. The shRNA with ITGB2 knockdown was constructed and transfected into ACHN cells for functional experiments to detect its effect on the malignant biological behaviors of ACHN cells, and its effect on the cell cycle was detected by flow cytometry. WB was used to detect the effect of ITGB2 knockdown on ITGB2 protein expression in ACHN cells. Results: The relative expression of ITGB2 in RCC tissues was significantly higher than that in para-cancerous tissue (P<0.01), and the expression was related to the clinical stage of RCC (P<0.05). Transfection of shITGB2 into ACHN cells could knock down the gene and protein expression of ITGB2 (all P<0.01). Knockdown of ITGB2 could significantly inhibit the proliferation (P<0.05), migration(P<0.01) and invasion (P<0.05) of ACHN cells but had no significant effect on cell cycle (P>0.05). Conclusion: ITGB2 is highly expressed in RCC tissues and cells and is associated with the clinical stage of RCC. Knockdown of ITGB2 can inhibit the malignant biological behaviors of ACHN cells.
- Full text:20220508.pdf
