Application of oral fluid in SARS-CoV-2 nucleic acid and antibody detection.
10.3760/cma.j.cn112150-20211211-01146
- Author:
Qi HUANG
1
;
Fang Yao LIU
2
;
Nai Ying MAO
3
;
Jing Yi SUN
4
;
Mei DONG
5
;
Hui XIE
5
;
Feng LIU
2
;
Heng ZHANG
2
;
Xia Li YU
5
;
Jian Ping DONG
4
;
Wenbo XU
3
;
Fang HUANG
1
Author Information
1. School of Public Health, Capital Medical University, Beijing 100069, China Institute of Preventive Immunization, Beijing Center for Disease Control and Prevention/Beijing Academy for Preventive Medicine/Beijing Institute of Tuberculosis Control Research and Prevention, Beijing 100013, China.
2. Department of Microbiology Laboratory, Beijing Haidian Center for Disease Control and Prevention, Beijing 100094, China.
3. Institute for Viral Disease Control and Prevention, Chinese Centers for Disease control and Prevention, Beijing 102206, China NHC Key Laboratory for Medical Virology and Viral Diseases, Beijing 102206, China.
4. Department Infectious Disease, Beijing Haidian Hospital, Beijing 100080, China.
5. Institute of Preventive Immunization, Beijing Center for Disease Control and Prevention/Beijing Academy for Preventive Medicine/Beijing Institute of Tuberculosis Control Research and Prevention, Beijing 100013, China.
- Publication Type:Journal Article
- MeSH:
COVID-19/diagnosis*;
Humans;
Nucleic Acids;
SARS-CoV-2;
Sensitivity and Specificity
- From:Chinese Journal of Preventive Medicine
2022;56(3):355-359
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to explore the application value of new biological specimen oral fluid in SARS-CoV-2 nucleic acid and antibody detection. Oral fluid and paired respiratory and blood specimens from 7 confirmed cases of two COVID-19 cluster epidemic were collected in Beijing from October to November 2021. SARS-CoV-2 virus and IgG antibody were detected by real time PCR kits and serum antibody detection reagents, and SARS-CoV-2 IgG antibody in oral fluids was detected by a new established method of magnetic particle chemiluminescence. The results showed that the nucleic acid amplification test of SARS-CoV-2 on nasopharyngeal swabs, throat swabs and oral fluid specimens from 3 confirmed cases of COVID-19 was positive, among which the Ct value for ORF1a/b and N gene of oral fluid samples in 2 cases was close to that of throat swab, and the Ct value of oral fluid sample for 1 case was higher than that of throat swab. The complete genome sequence of one oral fluid specimen was obtained, which belonged to the VOC/Delta variant strain. The SARS-CoV-2 IgG antibodies of the paired oral fluid and serum were all positive, and the S/CO values of oral fluid were all lower than those of serum. The series of oral fluid results showed that SARS-CoV-2 IgG antibody level increased from 11 to 32 days after the onset of the disease.
