Metabolomic study on clinical staging of methamphetamine detoxification by LC-QTOF-MS
10.11665/j.issn.1000-5048.20220309
- VernacularTitle:基于LC-QTOF-MS代谢组学研究甲基苯丙胺临床戒毒分期
- Author:
Jingqi WANG
1
;
Ye WANG
;
Yi ZHANG
;
Juncheng LIANG
;
Yanping DENG
;
Taijun HANG
;
Min SONG
Author Information
1. 中国药科大学药学院
- Publication Type:Journal Article
- Keywords:
methamphetamine;
LC-QTOF-MS;
multivariate analysis;
biomarker;
clinical stage of detoxification
- From:
Journal of China Pharmaceutical University
2022;53(3):314-322
- CountryChina
- Language:Chinese
-
Abstract:
At present , methamphetamine has become a major hidden danger in global public health safety. In order to judge methamphetamine addicts and methamphetamine abstainers more scientifically and reliably, this study analyzed the endogenous metabolites in plasma, serum and urine of methamphetamine addicts, methamphetamine abstainers and healthy volunteers by highly sensitive high-throughput liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) analytical instrument. The obtained metabolomic data were processed by univariate analysis (t-test) and multivariate analysis (PLS-DA and OPLS-DA) and eligible potential biomarkers were then screened.The identified biomarkers set enrichment analysis to find the connection between metabolites and metabolic pathways.Multivariate statistical results showed that methamphetamine acute group, recovery group and healthy group were clearly separated.3, 18 and 6 regulated metabolites were identified in serum, plasma and urine, respectively, suggesting that lipid metabolism was abnormal in methamphetamine acute group, and that fatty acid metabolism, sulfate/sulfite metabolism and sex hormone metabolism were abnormal in methamphetamine recovery group.The selected potential biomarkers in this study provide the possibility for scientific judgment of the clinical stage of methamphetamine detoxification.
