Effect of Qinggan Xiefei Fang on increasing susceptibility of influenza virus caused by "liver fire invading lung"
10.16438/j.0513-4870.2022-0254
- VernacularTitle:清肝泻肺方治疗肝火犯肺增加流感病毒易感性的作用研究
- Author:
Yu-hui LU
1
,
2
,
3
;
Shu-hua OUYANG
1
,
2
,
3
;
Jing-yu WENG
1
,
2
,
3
;
Pei LIU
1
,
4
;
Xin-xing CHEN
1
,
2
,
3
;
Kurihara HIROSHI
1
,
2
,
3
;
Yi-fang LI
1
,
2
,
3
;
Rong-rong HE
1
,
2
,
3
Author Information
1. Guangdong Engineering Research Center of Chinese Medicine and Disease Susceptibility, Jinan University, Guangzhou 510632, China
2. Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China
3. Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou 510632, China
4. School of Pharmacy and State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau 999078, China
- Publication Type:Research Article
- Keywords:
emotional stress;
Qinggan Xiefei Fang;
influenza viral pneumonia;
oxidative stress;
phospholipid peroxidation
- From:
Acta Pharmaceutica Sinica
2022;57(6):1649-1656
- CountryChina
- Language:Chinese
-
Abstract:
In this study, chronic emotional stress-induced H1N1 influenza susceptibility model was employed to simulate the states of "emotional stagnation" and "liver fire invading lung", and the protective effect of Qinggan Xiefei Fang on viral pneumonia was investigated. Survival rate and morbidity rate of mice were observed within 21 days after H1N1 infection, the symptoms of viral pneumonia and the level of phospholipid peroxidation were detected in lungs of mice after 6-day infection. The experimental results showed that Qinggan Xiefei Fang could alleviate the decline of survival rate and morbidity rate of mice caused by chronic constraint stress loaded with H1N1, inhibit the replication of H1N1 and the production of inflammatory factors, reduce the level of phospholipid peroxidation, and improve the symptoms of pneumonia in mice. The results also showed that compound-target network of Qinggan Xiefei Fang contained 171 compounds and 260 corresponding targets involved in the signaling pathway of oxidative stress, inflammation and immunity. All the above results indicate that Qinggan Xiefei Fang protecting influenza virus pneumonia was related to the regulation of oxidative stress. The animal experimental protocol has been reviewed and approved by Laboratory Animal Ethics Committee of Jinan University, in compliance with the Institutional Animal Care Guidelines.
