One-step synthesis of site-specific antibody-drug conjugates by reprograming IgG glycoengineering with LacNAc-based substrates.
10.1016/j.apsb.2021.12.013
- Author:
Wei SHI
1
;
Wanzhen LI
2
;
Jianxin ZHANG
2
;
Tiehai LI
1
;
Yakai SONG
1
;
Yue ZENG
1
;
Qian DONG
1
;
Zeng LIN
1
;
Likun GONG
1
;
Shuquan FAN
3
;
Feng TANG
1
;
Wei HUANG
1
Author Information
1. CAS Key Laboratory of Receptor Research, CAS Center for Excellence in Molecular Cell Science, Center for Biotherapeutics Discovery Research, Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai 201203, China.
2. School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China.
3. School of Life Science, Liaocheng University, Liaocheng 252059, China.
- Publication Type:Journal Article
- Keywords:
ENGase;
LacNAc;
One-step assembly;
Potent in vivo efficacy;
Site-specific ADCs
- From:
Acta Pharmaceutica Sinica B
2022;12(5):2417-2428
- CountryChina
- Language:English
-
Abstract:
Glycosite-specific antibody‒drug conjugatess (gsADCs), harnessing Asn297 N-glycan of IgG Fc as the conjugation site for drug payloads, usually require multi-step glycoengineering with two or more enzymes, which limits the substrate diversification and complicates the preparation process. Herein, we report a series of novel disaccharide-based substrates, which reprogram the IgG glycoengineering to one-step synthesis of gsADCs, catalyzed by an endo-N-acetylglucosaminidase (ENGase) of Endo-S2. IgG glycoengineering via ENGases usually has two steps: deglycosylation by wild-type (WT) ENGases and transglycosylation by mutated ENGases. But in the current method, we have found that disaccharide LacNAc oxazoline can be efficiently assembled onto IgG by WT Endo-S2 without hydrolysis of the product, which enables the one-step glycoengineering directly from native antibodies. Further studies on substrate specificity revealed that this approach has excellent tolerance on various modification of 6-Gal motif of LacNAc. Within 1 h, one-step synthesis of gsADC was achieved using the LacNAc-toxin substrates including structures free of bioorthogonal groups. These gsADCs demonstrated good homogeneity, buffer stability, in vitro and in vivo anti-tumor activity. This work presents a novel strategy using LacNAc-based substrates to reprogram the multi-step IgG glycoengineering to a one-step manner for highly efficient synthesis of gsADCs.
