A NIR fluorescent probe for Vanin-1 and its applications in imaging, kidney injury diagnosis, and the development of inhibitor.
10.1016/j.apsb.2021.06.004
- Author:
Zhenhao TIAN
1
;
Fei YAN
1
;
Xiangge TIAN
1
;
Lei FENG
1
;
Jingnan CUI
2
;
Sa DENG
1
;
Baojing ZHANG
1
;
Tian XIE
3
;
Shanshan HUANG
1
;
Xiaochi MA
1
Author Information
1. Dalian Key Laboratory of Metabolic Target Characterization and Traditional Chinese Medicine Intervention, The Second Affiliated Hospital, College of Integrative Medicine, College of Pharmacy, Dalian Medical University, Dalian 116044, China.
2. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
3. College of Pharmacy, Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Hangzhou Normal University, Hangzhou 311121, China.
- Publication Type:Journal Article
- Keywords:
Fluorescence imaging;
Kidney injury diagnosis;
NIR fluorescent probe;
Oleuropein;
Vanin-1
- From:
Acta Pharmaceutica Sinica B
2022;12(1):316-325
- CountryChina
- Language:English
-
Abstract:
Vanin-1 is an amidohydrolase that catalyses the conversion of pantetheine into the amino-thiol cysteamine and pantothenic acid (coenzyme A precursor), which plays a vital role in multiple physiological and pathological processes. In this study, an enzyme-activated near-infrared (NIR) fluorescent probe (DDAV) has been constructed for sensitively detecting Vanin-1 activity in complicated biosamples on the basis of its catalytic characteristics. DDAV exhibited a high selectivity and sensitivity toward Vanin-1 and was successfully applied to the early diagnosis of kidney injury in cisplatin-induced kidney injury model. In addition, DDAV could serve as a visual tool for in situ imaging endogenous Vanin-1 in vivo. More importantly, Enterococcus faecalis 20247 which possessed high expression of Vanin-1 was screened out from intestinal bacteria using DDAV, provided useful guidance for the rational use of NSAIDs in clinic. Finally, oleuropein as a potent natural inhibitor for Vanin-1 was discovered from herbal medicines library using a high-throughput screening method using DDAV, which held great promise for clinical therapy of inflammatory bowel disease.
