Identification of Andrographis Herba and its common products using mini-barcode.
10.1016/S1875-5364(22)60157-2
- Author:
Chao XIONG
1
,
2
;
Chen-Hao HUANG
3
,
4
;
Lan WU
5
;
Ran XU
6
;
Jian-Ping XUE
7
;
Zhi-Guo LIU
8
;
Wei SUN
9
Author Information
1. School of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
4. College of Life Sciences, Huaibei Normal University, Huaibei 235000, China.
5. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
6. School of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China.
7. College of Life Sciences, Huaibei Normal University, Huaibei 235000, China.
8. School of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: murielle.zhiguo_l@126.com.
9. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: murielle.wsun@icmm.cn.
- Publication Type:Journal Article
- Keywords:
Andrographis Herba;
Andrographis paniculata;
DNA barcoding;
Molecular identification
- MeSH:
Andrographis;
Andrographis paniculata;
DNA Primers;
Drugs, Chinese Herbal
- From:
Chinese Journal of Natural Medicines (English Ed.)
2022;20(5):393-400
- CountryChina
- Language:English
-
Abstract:
Andrographis Herba, the aerial part of Andrographis paniculata (Burm. f.) Wall. ex Nees (Acanthaceae), has a wide geographic distribution and has been used for the treatment of fever, cold, inflammation, and other infectious diseases. In markets, sellers and buyers commonly inadvertently confuse with related species. In addition, most Chinese medicinal herbs are subjected to traditional processing procedures, such as steaming and boiling, before they are sold at dispensaries; therefore, it is very difficult to identify Andrographis Herba when it is processed into Chinese medicines. The identification of species and processed medicinal materials is a growing issue in the marketplace. However, conventional methods of identification have limitations, while DNA barcoding has received considerable attention as a new potential means to identify species and processed medicinal materials. In this study, 17 standard reference materials of A. paniculata, 2 standard decoctions, 27 commercial products and two adulterants were collected. Based on the ITS2 sequence, it could successfully identify A. paniculata and adulterants. Moreover, a nucleotide signature consisting of 71 bp was designed, this sequence is highly conserved and specific within A. paniculata while divergent among other species. Then, we used these new primers to amplify the nucleotide signature region from processed materials. In conclusion, the DNA barcoding method developed in the present study for authenticating A. paniculata is rapid and cost-effective. It can be used in the future to guarantee the quality of Andrographis Herba of each regulatory link for clinical use.
