Anti-inflammatory effects of Abelmoschus manihot (L.) Medik. on LPS-induced cystitis in mice: potential candidate for cystitis treatment based on classic use.
10.1016/S1875-5364(22)60140-7
- Author:
Su ZHOU
1
;
Kai-Kai FAN
1
;
Li-Fei GU
1
;
Bo-Yang YU
2
,
3
;
Cheng-Zhi CHAI
4
Author Information
1. Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
2. Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China
3. Research Center for Traceability and Standardization of TCMs, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. Electronic address: murielle.boyangyu59@163.com.
4. Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. Electronic address: murielle.chengzhichai@cpu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Abelmoschus manihot (L.) Medik. (A. manihot);
Cystitis;
Inflammation;
Lipopolysaccharide (LPS);
TLR4/MYD88/NF-κB
- MeSH:
Abelmoschus/metabolism*;
Animals;
Anti-Inflammatory Agents/therapeutic use*;
Cystitis;
Female;
Humans;
Lipopolysaccharides/pharmacology*;
Male;
Mice;
Myeloid Differentiation Factor 88/metabolism*;
NF-KappaB Inhibitor alpha/metabolism*;
NF-kappa B/metabolism*;
Signal Transduction;
Toll-Like Receptor 4/metabolism*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2022;20(5):321-331
- CountryChina
- Language:English
-
Abstract:
Abelmoschus manihot (L.) Medik. (A. manihot) is a traditional Chinese herbal medicine with a variety of pharmacological properties. It was first recorded in Jiayou Materia Medica dating back to the Song dynasty to eliminate urinary tract irritation by clearing away heat and diuretic effect. However, its pharmacological action on urinary tract infections has not been investigated. The present study aims to evaluate the anti-inflammatory activity of A. manihot on a mouse model of lipopolysaccharide (LPS)-induced cystitis. The results showed that A. manihot decreased white blood cell (WBC) count in urine sediments of the cystitis mice, alleviated bladder congestion, edema, as well as histopathological damage, reduced the expression levels of tumor necrosis factor-α, interleukin-6, and interleukin-1β simultaneously. Moreover, A. manihot administration significantly downregulated the expression levels of TLR4, MYD88, IκBα, p-IκBα, NF-κB p65, and p-NF-κB p65 in LPS-induced cystitis mice. These findings demonstrated the protective effect of A. manihot against LPS-induced cystitis, which is attributed to its anti-inflammatory profile by suppressing TLR4/MYD88/NF-κB pathways. Our results suggest that A. manihot could be a potential candidate for cystitis treatment.
