Integrating 16S sequencing and metabolomics study on anti-rheumatic mechanisms against collagen-induced arthritis of Wantong Jingu Tablet.
10.1016/S1875-5364(21)60080-8
- Author:
Zhao-Dong LI
1
;
Fang-Yuan QI
1
;
Fan LI
2
,
3
,
4
,
5
,
6
Author Information
1. Department of Pathogenobiology, the Key Laboratory of Zoonosis, Ministry of Education of China, School of Basic Medicine, Jilin University, Changchun 130000, China.
2. Department of Pathogenobiology, the Key Laboratory of Zoonosis, Ministry of Education of China, School of Basic Medicine, Jilin University, Changchun 130000, China
3. Key Laboratory for Bionics Engineering, Ministry of Education of China, Jilin University, Changchun 130000, China
4. Engineering Research Center for Medical Biomaterials of Jilin Province, Jilin University, Changchun 130000, China
5. Key Laboratory for Biomedical Materials of Jilin Province, Jilin University, Changchun 130000, China
6. State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Urumqi 830000, China. Electronic address: lifan@jlu.edu.cn.
- Publication Type:Journal Article
- Keywords:
16S rDNA sequencing;
Metabolomics;
Pathogenesis;
Rheumatoid arthritis;
Wantong Jingu Tablet
- MeSH:
Animals;
Arthritis, Experimental/drug therapy*;
Arthritis, Rheumatoid/drug therapy*;
Dysbiosis;
Metabolomics;
Rats;
Tablets
- From:
Chinese Journal of Natural Medicines (English Ed.)
2022;20(2):120-132
- CountryChina
- Language:English
-
Abstract:
Wantong Jingu Tablet (WJT), a mixture of traditional Chinese medicine, was reported to relieve the symptoms of rheumatoid arthritis (RA), but its pharmacological mechanism was not completely understood. The aim of this study was to investigate the therapeutic mechanisms of WJT for RA in vivo. The effects of WJT on joint pathology, as well as the levels of Bax, Bcl-2, caspase-3, cleaved-caspase-3, ERK1/2, pERK1/2, TNF-α, IL-1β, and IL-6 were measured using collagen-induced arthritis (CIA) rats. The intestinal flora composition and the metabolites alteration were analyzed by 16S rDNA sequencing and metabolomics method, respectively. We found that WJT ameliorated the severity of the CIA rats which might be mediated by inducing apoptosis, inactivating the MEK/ERK signals and reducing the production of pro-inflammatory cytokines. WJT, in part, relieved the gut microbiota dysbiosis, especially bacterial phylum Bacteroidetes, Tenericutes and Deferribacteres, as well as bacterial genus Vibrio, Macrococcus and Vagococcus. 3'-N-debenzoyl-2'-deoxytaxol, tubulysin B, and magnoline were significantly associated with the specific genera. We identified serotonin, glutathione disulfide, N-acetylneuraminic acid, naphthalene and thromboxane B2 as targeted molecules via metabolomics. Our findings contributed to the understanding of RA pathogenesis, and WJT played essential roles in gut microbiota health and metabolite modulation in the CIA rats.
