Novel mutation c.1210-3C > G in cis with a poly-T tract of 5T affects CFTR mRNA splicing in a Chinese patient with cystic fibrosis.
10.1007/s11684-021-0846-5
- Author:
Xinyue ZHAO
1
;
Keqiang LIU
1
;
Wenshuai XU
2
;
Meng XIAO
1
;
Qianli ZHANG
1
;
Jiaxing SONG
1
;
Keqi CHEN
2
;
Yaping LIU
3
;
Xinlun TIAN
4
;
Kai-Feng XU
2
;
Xue ZHANG
1
Author Information
1. McKusick-Zhang Center for Genetic Medicine, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China.
2. Department of Pulmonary and Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China.
3. McKusick-Zhang Center for Genetic Medicine, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China. ypliu@ibms.pumc.edu.cn.
4. Department of Pulmonary and Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China. xinlun_t@sina.com.
- Publication Type:Journal Article
- Keywords:
CFTR;
cystic fibrosis;
minigene;
splicing mutation
- MeSH:
China;
Cystic Fibrosis/genetics*;
Cystic Fibrosis Transmembrane Conductance Regulator/genetics*;
Humans;
Mutation;
Poly T;
RNA, Messenger/genetics*
- From:
Frontiers of Medicine
2022;16(1):150-155
- CountryChina
- Language:English
-
Abstract:
Cystic fibrosis (CF) is a rare autosomal recessive disease with only one pathogenic gene cystic fibrosis transmembrane conductance regulator (CFTR). To identify the potential pathogenic mutations in a Chinese patient with CF, we conducted Sanger sequencing on the genomic DNA of the patient and his parents and detected all 27 coding exons of CFTR and their flanking intronic regions. The patient is a compound heterozygote of c.2909G > A, p.Gly970Asp in exon 18 and c.1210-3C > G in cis with a poly-T of 5T (T5) sequence, 3 bp upstream in intron 9. The splicing effect of c.1210-3C > G was verified via minigene assay in vitro, indicating that wild-type plasmid containing c.1210-3C together with T7 sequence produced a normal transcript and partial exon 10-skipping-transcript, whereas mutant plasmid containing c.1210-3G in cis with T5 sequence caused almost all mRNA to skip exon 10. Overall, c.1210-3C > G, the newly identified pathogenic mutation in our patient, in combination with T5 sequence in cis, affects the CFTR gene splicing and produces nearly no normal transcript in vitro. Moreover, this patient carries a p.Gly970Asp mutation, thus confirming the high-frequency of this mutation in Chinese patients with CF.
