The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication.
10.1007/s13238-021-00822-1
- Author:
Wan ZHAO
1
;
Junjie ZHU
1
;
Hong LU
1
;
Jiaming ZHU
1
;
Fei JIANG
1
;
Wei WANG
1
;
Lan LUO
1
;
Le KANG
1
;
Feng CUI
2
Author Information
1. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
2. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. cuif@ioz.ac.cn.
- Publication Type:Research Support, Non-U.S. Gov't
- Keywords:
YY1;
importin α;
nuclear localization;
nucleocapsid protein;
rice stripe virus
- MeSH:
Animals;
Cell Nucleus;
Hemiptera/metabolism*;
Insect Vectors/genetics*;
Insecta;
Nucleocapsid Proteins/metabolism*;
Oryza;
Plant Diseases;
Tenuivirus/metabolism*;
Virus Replication
- From:
Protein & Cell
2022;13(5):360-378
- CountryChina
- Language:English
-
Abstract:
Rice stripe virus (RSV) transmitted by the small brown planthopper causes severe rice yield losses in Asian countries. Although viral nuclear entry promotes viral replication in host cells, whether this phenomenon occurs in vector cells remains unknown. Therefore, in this study, we systematically evaluated the presence and roles of RSV in the nuclei of vector insect cells. We observed that the nucleocapsid protein (NP) and viral genomic RNAs were partially transported into vector cell nuclei by utilizing the importin α nuclear transport system. When blocking NP nuclear localization, cytoplasmic RSV accumulation significantly increased. In the vector cell nuclei, NP bound the transcription factor YY1 and affected its positive regulation to FAIM. Subsequently, decreased FAIM expression triggered an antiviral caspase-dependent apoptotic reaction. Our results reveal that viral nuclear entry induces completely different immune effects in vector and host cells, providing new insights into the balance between viral load and the immunity pressure in vector insects.
