MouseVenue3D: A Markerless Three-Dimension Behavioral Tracking System for Matching Two-Photon Brain Imaging in Free-Moving Mice.
10.1007/s12264-021-00778-6
- Author:
Yaning HAN
1
;
Kang HUANG
1
;
Ke CHEN
1
;
Hongli PAN
1
;
Furong JU
1
;
Yueyue LONG
1
;
Gao GAO
1
;
Runlong WU
2
;
Aimin WANG
3
;
Liping WANG
4
;
Pengfei WEI
5
Author Information
1. Shenzhen Key Laboratory of Neuropsychiatric Modulation and Collaborative Innovation Center for Brain Science, Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Center for Excellence in Brain Science and Intelligence Technology, Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen-Hong Kong Institute of Brain Science, Shenzhen Fundamental Research Institutions, Shenzhen, 518055, China.
2. State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking University, Beijing, 100101, China.
3. Department of Electronics, Peking University, Beijing, 100871, China.
4. Shenzhen Key Laboratory of Neuropsychiatric Modulation and Collaborative Innovation Center for Brain Science, Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Center for Excellence in Brain Science and Intelligence Technology, Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen-Hong Kong Institute of Brain Science, Shenzhen Fundamental Research Institutions, Shenzhen, 518055, China. lp.wang@siat.ac.cn.
5. Shenzhen Key Laboratory of Neuropsychiatric Modulation and Collaborative Innovation Center for Brain Science, Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Center for Excellence in Brain Science and Intelligence Technology, Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen-Hong Kong Institute of Brain Science, Shenzhen Fundamental Research Institutions, Shenzhen, 518055, China. pf.wei@siat.ac.cn.
- Publication Type:Journal Article
- Keywords:
3-D pose estimation;
Automatic calibration;
Behavioral and neural recording;
Computational neuroethology;
Multi-view cameras
- MeSH:
Animals;
Behavior, Animal;
Brain/diagnostic imaging*;
Imaging, Three-Dimensional/methods*;
Mice;
Neuroimaging;
Rodentia
- From:
Neuroscience Bulletin
2022;38(3):303-317
- CountryChina
- Language:English
-
Abstract:
Understanding the connection between brain and behavior in animals requires precise monitoring of their behaviors in three-dimensional (3-D) space. However, there is no available three-dimensional behavior capture system that focuses on rodents. Here, we present MouseVenue3D, an automated and low-cost system for the efficient capture of 3-D skeleton trajectories in markerless rodents. We improved the most time-consuming step in 3-D behavior capturing by developing an automatic calibration module. Then, we validated this process in behavior recognition tasks, and showed that 3-D behavioral data achieved higher accuracy than 2-D data. Subsequently, MouseVenue3D was combined with fast high-resolution miniature two-photon microscopy for synchronous neural recording and behavioral tracking in the freely-moving mouse. Finally, we successfully decoded spontaneous neuronal activity from the 3-D behavior of mice. Our findings reveal that subtle, spontaneous behavior modules are strongly correlated with spontaneous neuronal activity patterns.
