Efficient genetic transformation and CRISPR/Cas9-mediated genome editing of watermelon assisted by genes encoding developmental regulators.
- Author:
Wenbo PAN
1
;
Zhentao CHENG
2
;
Zhiguo HAN
2
;
Hong YANG
2
;
Wanggen ZHANG
3
;
Huawei ZHANG
4
Author Information
- Publication Type:Journal Article
- MeSH: CRISPR-Cas Systems; Citrullus/genetics*; Cucurbitaceae/genetics*; Gene Editing; Plant Breeding; Transformation, Genetic
- From: Journal of Zhejiang University. Science. B 2022;23(4):339-344
- CountryChina
- Language:English
- Abstract: Cucurbitaceae is an important family of flowering plants containing multiple species of important food plants, such as melons, cucumbers, squashes, and pumpkins. However, a highly efficient genetic transformation system has not been established for most of these species (Nanasato and Tabei, 2020). Watermelon (Citrullus lanatus), an economically important and globally cultivated fruit crop, is a model species for fruit quality research due to its rich diversity of fruit size, shape, flavor, aroma, texture, peel and flesh color, and nutritional composition (Guo et al., 2019). Through pan-genome sequencing, many candidate loci associated with fruit quality traits have been identified (Guo et al., 2019). However, few of these loci have been validated. The major barrier is the low transformation efficiency of the species, with only few successful cases of genetic transformation reported so far (Tian et al., 2017; Feng et al., 2021; Wang JF et al., 2021; Wang YP et al., 2021). For example, Tian et al. (2017) obtained only 16 transgenic lines from about 960 cotyledon fragments, yielding a transformation efficiency of 1.67%. Therefore, efficient genetic transformation could not only facilitate the functional genomic studies in watermelon as well as other horticultural species, but also speed up the transgenic and genome-editing breeding.
