Genetic diversity analysis of forty-three insertion/deletion loci for forensic individual identification in Han Chinese from Beijing based on a novel panel.

Congying Zhao; Jinlong Yang; Hui XU; Shuyan Mei; Yating Fang; Qiong Lan; Yajun Deng; Bofeng Zhu

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Genetic diversity analysis of forty-three insertion/deletion loci for forensic individual identification in Han Chinese from Beijing based on a novel panel.

Author: Congying ZHAO ¹ ; Jinlong YANG ² ; Hui XU ¹ ; Shuyan MEI ¹ ; Yating FANG ¹ ; Qiong LAN ¹ ; Yajun DENG ³ ; Bofeng ZHU ⁴
Author Information

1. Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China.
2. Beijing Zhongzheng DNA Evidence Institute, Beijing 101318, China.
3. Beijing Zhongzheng DNA Evidence Institute, Beijing 101318, China. zhubofeng7372@126.com, 18601285369@163.com.
4. Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China. zhubofeng7372@126.com.
Publication Type:Journal Article
Keywords: Chinese Beijing Han; Human identification; InDel; Population genetics
MeSH: Beijing; China; Forensic Genetics/methods*; Gene Frequency; Genetics, Population; Humans; INDEL Mutation; Phylogeny
From: Journal of Zhejiang University. Science. B 2022;23(3):241-248
CountryChina
Language:English
Abstract: Due to the virtues of no stutter peaks, low rates of mutation, and short amplicon sizes, insertion/deletion (InDel) polymorphism is an indispensable tool for analyzing degraded DNA samples from crime scenes for human identifications (Wang et al., 2021). Herein, a self-developed panel of 43 InDel loci constructed previously by our group was utilized to evaluate the genetic diversities and explore the genetic background of the Han Chinese from Beijing (HCB) including 301 random healthy individuals. The lengths of amplicons at 43 InDel loci in this panel ranged from 87 to 199 bp, which indicated that the panel could be used as an effective tool to utilize highly degraded DNA samples for human identity testing. The loci in this panel were validated and performed well for forensic degraded DNA samples (Jin et al., 2021). The combined discrimination power (PD) and combined probability of exclusion (PE) values in this panel indicated that the 43 InDel loci could be used as the candidate markers in personal identification and parentage testing of HCB. In addition, population genetic relationships between the HCB and 26 reference populations from five continents based on 19 overlapped InDel loci were displayed by constructing a phylogenetic tree, principal component analysis (PCA), and population genetic structure analysis. The results illustrated that the HCB had closer genetic relationships with the Han populations from Chinese different regions.