Chemical characterization of a new sulfated polysaccharide from <i>Gracilaria chouae</i> and its activation effects on RAW264.7 macrophages.

Feifei LI; Kehai Liu; Kewu Liu

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Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages.

Author: Feifei LI ¹ ; Kehai LIU ² ; Kewu LIU ³
Author Information

1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. khliu@shou.edu.cn.
3. Heilongjiang Forest By-product and Speciality Institute, Mudanjiang 157011, China.
Publication Type:Journal Article
MeSH: Gracilaria/chemistry*; Macrophages; Molecular Weight; Polysaccharides/pharmacology*; Sulfates/pharmacology*
From: Journal of Zhejiang University. Science. B 2022;23(1):84-88
CountryChina
Language:English
Abstract: This study aimed to characterize the chemical composition of a new sulfated polysaccharide from the red alga Gracilaria chouae and evaluate its activation effects on RAW264.7 macrophages. It showed that the obtained G. chouae polysaccharide (GCP-3A) was a sulfated acidic polysaccharide with a molecular weight of 11.87 kDa. GCP-3A was composed of xylose, galactose, glucose, and mannose with a molar ratio of 3.00:29.28:0.63:0.45, and it contained α,β‍-glycosidic linkages. Scanning electron microscopy (SEM) and a Congo red test showed that it was a heterogeneous polysaccharide with irregular interwoven sheets and rods, and did not have a triple-helix conform‍ation. Furthermore, GCP-3A significantly promoted the proliferation of RAW264.7 macrophages and the secretion of nitric oxide (NO) in tests of 3-‍(4,‍5-dimethylthiahiazo-2-yl)‍-2,‍5-diphenytetrazoliumromide(MTT) and NO.