Dissimilarity of ccrAB gene sequences between methicillin-resistant Staphylococcus epidermidis and methicillin-resistant Staphylococcus aureus among bovine isolates in Korea.
10.4142/jvs.2013.14.3.299
- Author:
Young Kyung PARK
1
;
Young Hwan PAIK
;
Jang Won YOON
;
Lawrence K FOX
;
Sun Young HWANG
;
Yong Ho PARK
Author Information
1. Department of Microbiology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea. yhp2738@korea.kr
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
ccrAB;
methicillin-resistant Staphylococcus aureus;
methicillin-resistant Staphylococcus epidermidis;
SCCmec
- MeSH:
Animals;
Anti-Bacterial Agents/*pharmacology;
Bacterial Proteins/*genetics/metabolism;
Bacterial Typing Techniques/veterinary;
Cattle;
Cattle Diseases/epidemiology/metabolism;
Chickens;
Dog Diseases/epidemiology/metabolism;
Dogs;
*Drug Resistance, Bacterial;
*Gene Transfer, Horizontal;
Methicillin/*pharmacology;
Methicillin-Resistant Staphylococcus aureus/genetics/isolation & purification;
Milk/microbiology;
Multilocus Sequence Typing/veterinary;
Poultry Diseases/epidemiology/metabolism;
Prevalence;
Republic of Korea/epidemiology;
Staphylococcal Infections/epidemiology/microbiology/*veterinary;
Staphylococcus epidermidis/genetics/isolation & purification
- From:Journal of Veterinary Science
2013;14(3):299-305
- CountryRepublic of Korea
- Language:English
-
Abstract:
The sequences of the ccrAB genes from bovine-, canine- and chicken-originating methicillin-resistant Staphylococcus (S.) epidermidis (MRSE) and bovine methicillin-resistant Staphylococcus (S.) aureus (MRSA) were compared to investigate the frequency of intra-species horizontal transfer of the staphylococcal cassette chromosome mec (SCCmec) complex. Nineteen MRSE strains were isolated from bovine milk, chickens, and dogs, and their genetic characteristics were investigated by multilocus sequence typing and SCCmec typing. Among the animal MRSE strains, the most frequent SCCmec type was type IV, which consisted of the type B mec complex and ccrAB type 2. The ccrA2 and ccrB2 genes were sequenced from the bovine, chicken and canine MRSE strains and compared with those of the bovine MRSA strains. The sequences generally clustered as MRSA and MRSE groups, regardless of the animal source. Additionally, no bovine MRSE sequence was associated with the bovine MRSA groups. Although most of the bovine MRSE and MRSA isolates possessed SCCmec type IV sequences, our results suggest that the intra-species gene transfer of the SCCmec complex between bovine S. aureus and bovine S. epidermidis strains is not a frequent event.
