Improved rat spinal cord injury model using spinal cord compression by percutaneous method.
10.4142/jvs.2013.14.3.329
- Author:
Wook Hun CHUNG
1
;
Jae Hoon LEE
;
Dai Jung CHUNG
;
Wo Jong YANG
;
A Jin LEE
;
Chi Bong CHOI
;
Hwa Seok CHANG
;
Dae Hyun KIM
;
Hyo Jin CHUNG
;
Hyun Jung SUH
;
Soo Han HWANG
;
Hoon HAN
;
Sun Hee DO
;
Hwi Yool KIM
Author Information
1. Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea. hykim@konkuk.ac.kr
- Publication Type:Original Article ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords:
balloon compression;
laminectomy-free;
magnetic resonance imaging;
percutaneous spinal cord injury;
rat
- MeSH:
Animals;
Balloon Embolectomy/*methods;
Disease Models, Animal;
Male;
Rats;
Rats, Sprague-Dawley;
Spinal Cord Compression/*therapy
- From:Journal of Veterinary Science
2013;14(3):329-335
- CountryRepublic of Korea
- Language:English
-
Abstract:
Here, percutaneous spinal cord injury (SCI) methods using a balloon catheter in adult rats are described. A balloon catheter was inserted into the epidural space through the lumbosacral junction and then inflated between T9-T10 for 10min under fluoroscopic guidance. Animals were divided into three groups with respect to inflation volume: 20 microL (n = 18), 50 microL (n = 18) and control (Fogarty catheter inserted but not inflated; n = 10). Neurological assessments were then made based on BBB score, magnetic resonance imaging and histopathology. Both inflation volumes produced complete paralysis. Gradual recovery of motor function occurred when 20 microL was used, but not after 50 microL was applied. In the 50 microL group, all gray and white matter was lost from the center of the lesion. In addition, supramaximal damage was noted, which likely prevented spontaneous recovery. This percutaneous spinal cord compression injury model is simple, rapid with high reproducibility and the potential to serve as a useful tool for investigation of pathophysiology and possible protective treatments of SCI in vivo.
