Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B.
10.1007/s11655-021-3507-2
- Author:
Qian-Qian WANG
1
;
Meng WANG
1
;
Yan LI
2
;
Yong-Hui LIU
1
;
Lian-Qing SUN
3
Author Information
1. Department of Traditional Chinese Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China.
2. Department of Peripheral Vascular, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China.
3. Department of Traditional Chinese Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China. sunlianqing1@126.com.
- Publication Type:Journal Article
- Keywords:
apoptosis;
hyperglycemia;
pyroptosis;
salvianolic acid B
- MeSH:
Apoptosis;
Benzofurans/pharmacology*;
Oxidative Stress;
Pyroptosis;
Reactive Oxygen Species/metabolism*
- From:
Chinese journal of integrative medicine
2022;28(3):243-248
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To determine whether salvianolic acid B (Sal B) exerts protective effects on diabetic peripheral neuropathy by attenuating apoptosis and pyroptosis.
METHODS:RSC96 cells were primarily cultured with DMEM (5.6 mmol/L glucose), hyperglycemia (HG, 125 mmol/L glucose) and Sal B (0.1, 1, and 10 µ mol/L). Cells proliferation was measured by 3-(4, 5-cimethylthiazol-2-yl)-2, 5-dilphenyltetrazolium bromide assay. Reactive oxygen species (ROS) generation and apoptosis rate were detected by flow cytometry analysis. Western blot was performed to analyze the expressions of poly ADP-ribose polymerase (PARP), cleaved-caspase 3, cleaved-caspase 9, Bcl-2, Bax, NLRP3, ASC, and interleukin (IL)-1β.
RESULTS:Treatment with HG at a concentration of 125 mmol/L attenuated cellular proliferation, while Sal B alleviated this injury (P<0.05). In addition, Sal B inhibited HG-induced ROS production and apoptosis rate (P<0.05). Furthermore, treatment with Sal B down-regulated HG-induced PARP, cleaved-caspase 3, cleaved-caspase 9, Bax, NLRP3, ASC, and IL-1β expression, but mitigated HG-mediated down-regulation of Bcl-2 expression (P<0.05).
CONCLUSION:Sal B may protect RSC96 cells against HG-induced cellular injury via the inhibition of apoptosis and pyroptosis activated by ROS.
