Andrographolide Inhibits Proliferation and Promotes Apoptosis in Bladder Cancer Cells by Interfering with NF- κ B and PI3K/AKT Signaling In Vitro and In Vivo.
10.1007/s11655-022-3464-4
- Author:
Lei XUAN
1
;
Jing-Hai HU
1
;
Ran BI
1
;
Si-Qi LIU
1
;
Chun-Xi WANG
2
Author Information
1. Department of Urology, the First Hospital of Jilin University, Changchun, 130021, China.
2. Department of Urology, the First Hospital of Jilin University, Changchun, 130021, China. chunxi@jlu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
PI3K-AKT;
andrographolide;
bladder cancer;
nuclear factor κ B
- MeSH:
Animals;
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Diterpenes/therapeutic use*;
Humans;
Mice;
NF-kappa B/metabolism*;
Phosphatidylinositol 3-Kinases/metabolism*;
Proto-Oncogene Proteins c-akt/metabolism*;
Urinary Bladder Neoplasms/drug therapy*
- From:
Chinese journal of integrative medicine
2022;28(4):349-356
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To explore the influences of andrographolide (Andro) on bladder cancer cell lines and a tumor xenograft mouse model bearing 5637 cells.
METHODS:For in vitro experiments, T24 cells were stimulated with Andro (0-40 µmol/L) and 5637 cells were stimulated with Andro (0 to 80 µmol/L). Cell growth, migration, and infiltration were assessed using cell counting kit-8, colony formation, wound healing, and transwell assays. Apoptosis rate was examined using flow cytometry. In in vivo study, the antitumor effect of Andro (10 mg/kg) was evaluated by 5637 tumor-bearing mice, and levels of nuclear factor κ B (NF- κ B) and phosphoinositide 3-kinase/AKT related-proteins were determined by immunoblotting.
RESULTS:Andro suppressed growth, migration, and infiltraion of bladder cancer cells (P⩽0.05 or P⩽0.01). Additionally, Andro induced intrinsic mitochondria-dependent apoptosis in bladder cancer cell lines. Furthermore, Andro inhibited bladder cancer growth in mice (P⩽0.01). The expression of p65, p-AKT were suppressed by Andro treatment in vitro and in vivo (P⩽0.05 or P⩽0.01).
CONCLUSIONS:Andrographolide inhibits proliferation and promotes apoptosis in bladder cancer cells by interfering with NF- κ B and PI3K/AKT signaling in vitro and in vivo.
