Quantitative Proteomics Using Isobaric Labeling: A Practical Guide.
10.1016/j.gpb.2021.08.012
- Author:
Xiulan CHEN
1
,
2
;
Yaping SUN
1
,
3
;
Tingting ZHANG
1
,
3
;
Lian SHU
1
,
3
;
Peter ROEPSTORFF
4
;
Fuquan YANG
1
,
5
Author Information
1. Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2. University of Chinese Academy of Sciences, Beijing 100149, China. Electronic address: chenxiulan@moon.ibp.ac.cn.
3. University of Chinese Academy of Sciences, Beijing 100149, China.
4. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
5. University of Chinese Academy of Sciences, Beijing 100149, China. Electronic address: fqyang@ibp.ac.cn.
- Publication Type:Research Support, Non-U.S. Gov't
- Keywords:
Isobaric labeling;
Mass spectrometry;
Quantitative proteomics;
TMT;
iTRAQ
- MeSH:
Proteome/metabolism*;
Proteomics/methods*;
Reproducibility of Results;
Tandem Mass Spectrometry/methods*
- From:
Genomics, Proteomics & Bioinformatics
2021;19(5):689-706
- CountryChina
- Language:English
-
Abstract:
In the past decade, relative proteomic quantification using isobaric labeling technology has developed into a key tool for comparing the expression of proteins in biological samples. Although its multiplexing capacity and flexibility make this a valuable technology for addressing various biological questions, its quantitative accuracy and precision still pose significant challenges to the reliability of its quantification results. Here, we give a detailed overview of the different kinds of isobaric mass tags and the advantages and disadvantages of the isobaric labeling method. We also discuss which precautions should be taken at each step of the isobaric labeling workflow, to obtain reliable quantification results in large-scale quantitative proteomics experiments. In the last section, we discuss the broad applications of the isobaric labeling technology in biological and clinical studies, with an emphasis on thermal proteome profiling and proteogenomics.
